Linked Life Data

19409856

Source:http://linkedlifedata.com/resource/pubmed/id/19409856

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2009-6-29
pubmed:abstractText
Acupuncture is widely used clinically to treat acute and chronic pain conditions, but the mechanisms underlying its effect are not fully understood. Although endocannabinoids are involved in modulation of nociception in animal models and in humans, their role in acupuncture analgesia has not been assessed. In this report, we determined the effect of electroacupuncture (EA) on the level of anandamide in the skin tissue and the role of cannabinoid CB1 and CB2 receptors in the analgesic effect of EA in an animal model of inflammatory pain. Inflammatory pain was induced by local injection of complete Freund's adjuvant (CFA) into the hind paw of rats. Thermal hyperalgesia was tested with a radiant heat stimulus, and mechanical allodynia was quantified with von Frey filaments. The anandamide concentration in the skin tissue was measured by using high-performance liquid chromatography. EA, applied to GB30 and GB34, at 2 and 100Hz significantly reduced thermal hyperalgesia and mechanical allodynia induced by CFA injection. Compared with the sham group, EA significantly increased the anandamide level in the inflamed skin tissue. Local pretreatment with a specific CB2 receptor antagonist, AM630, significantly attenuated the antinociceptive effect of EA. However, the effect of EA was not significantly altered by AM251, a selective CB1 receptor antagonist. These findings suggest that EA potentiates the local release of endogenous anandamide from inflamed tissues. Activation of peripheral CB2 receptors contributes to the analgesic effect of EA on inflammatory pain. PERSPECTIVE: This study shows that electroacupuncture increases the anandamide level in inflammatory skin tissues, and CB2 receptors contribute to the analgesic effect of electroacupuncture in a rat model of inflammatory pain. This information improves our understanding of the mechanisms involved in the analgesic effect of acupuncture.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1528-8447
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
10
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
732-9
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:19409856-Animals, pubmed-meshheading:19409856-Arachidonic Acids, pubmed-meshheading:19409856-Chromatography, High Pressure Liquid, pubmed-meshheading:19409856-Electroacupuncture, pubmed-meshheading:19409856-Freund's Adjuvant, pubmed-meshheading:19409856-Hindlimb, pubmed-meshheading:19409856-Hot Temperature, pubmed-meshheading:19409856-Hyperalgesia, pubmed-meshheading:19409856-Indoles, pubmed-meshheading:19409856-Male, pubmed-meshheading:19409856-Pain, pubmed-meshheading:19409856-Pain Management, pubmed-meshheading:19409856-Physical Stimulation, pubmed-meshheading:19409856-Piperidines, pubmed-meshheading:19409856-Polyunsaturated Alkamides, pubmed-meshheading:19409856-Pyrazoles, pubmed-meshheading:19409856-Rats, pubmed-meshheading:19409856-Rats, Sprague-Dawley, pubmed-meshheading:19409856-Receptor, Cannabinoid, CB1, pubmed-meshheading:19409856-Receptor, Cannabinoid, CB2, pubmed-meshheading:19409856-Skin
pubmed:year
2009
pubmed:articleTitle
Endogenous anandamide and cannabinoid receptor-2 contribute to electroacupuncture analgesia in rats.
pubmed:affiliation
Department of Neurobiology, Tongji Medical College of Huazhong University of Science and Technology, Wuhan, Peoples Republic of China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't