Linked Life Data

19409377

Source:http://linkedlifedata.com/resource/pubmed/id/19409377

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
18
pubmed:dateCreated
2009-11-27
pubmed:abstractText
OBJECTIVES: Nowadays, nearly 1000 hemoglobin (Hb) variants are known. The standard biochemical techniques used in Hb analysis are mainly: isoelectric focusing, cation-exchange liquid chromatography (LC) and reversed-phase LC. In addition to this approach, a protein analysis is achieved by mass spectrometry (MS) and additional DNA studies are performed. The aim of this review is to emphasize the significance of MS methods applied to Hb variants analysis. RESULTS AND PERSPECTIVES: To perform Hb studies, different MS techniques are currently used such as electrospray ionization (ESI), matrix-assisted laser desorption ionization (MALDI) and tandem mass spectrometry (MS/MS). As shown here, MS is an efficient tool for identification of all types of variants (substitution of a single amino acid residue, several substitutions in the same globin chain, insertions/deletions, fusion Hbs). The use of MS in neonatal screening of Hb variants is also presented. CONCLUSIONS: MS is a powerful technique for Hb analysis. It appears as being an important additional method in the set of biochemical techniques.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1873-2933
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
42
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1807-17
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Phenotype determination of hemoglobinopathies by mass spectrometry.
pubmed:affiliation
Institut de Biologie et de Biochimie des Protéines, CNRS UMR 5086, IFR128, Université de Lyon, Lyon, France.
pubmed:publicationType
Journal Article, Review