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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1991-12-20
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pubmed:abstractText |
The movement protein of red clover necrotic mosaic virus (RCNMV) was expressed in Escherichia coli as a fusion with a maltose-binding protein using the vector pMAL-cRI and used to produce an antiserum. The RCNMV movement protein was detected in a cell wall fraction obtained from infected Nicotiana clevelandii leaf tissue by immunoblotting using the movement protein antiserum. The movement protein could be detected 6 h after inoculation and reached a maximum after 24 h. In contrast, the virus capsid protein, detected in a soluble fraction by immunoblotting using a capsid antiserum, continued to increase for 72 h after inoculation.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/ATP-Binding Cassette Transporters,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Escherichia coli Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Maltose-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Monosaccharide Transport Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/maltose transport system, E coli
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0022-1317
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
72 ( Pt 11)
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2853-6
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:1940875-ATP-Binding Cassette Transporters,
pubmed-meshheading:1940875-Base Sequence,
pubmed-meshheading:1940875-Carrier Proteins,
pubmed-meshheading:1940875-Cloning, Molecular,
pubmed-meshheading:1940875-DNA, Viral,
pubmed-meshheading:1940875-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:1940875-Escherichia coli,
pubmed-meshheading:1940875-Escherichia coli Proteins,
pubmed-meshheading:1940875-Fabaceae,
pubmed-meshheading:1940875-Immunoblotting,
pubmed-meshheading:1940875-Maltose-Binding Proteins,
pubmed-meshheading:1940875-Molecular Sequence Data,
pubmed-meshheading:1940875-Monosaccharide Transport Proteins,
pubmed-meshheading:1940875-Mosaic Viruses,
pubmed-meshheading:1940875-Plants, Medicinal,
pubmed-meshheading:1940875-Plants, Toxic,
pubmed-meshheading:1940875-Recombinant Fusion Proteins,
pubmed-meshheading:1940875-Tobacco,
pubmed-meshheading:1940875-Viral Proteins
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pubmed:year |
1991
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pubmed:articleTitle |
Detection of the movement protein of red clover necrotic mosaic virus in a cell wall fraction from infected Nicotiana clevelandii plants.
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pubmed:affiliation |
Department of Biology, Imperial College of Science, Technology and Medicine, London, U.K.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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