Source:http://linkedlifedata.com/resource/pubmed/id/19406778
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
Pt 5
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| pubmed:dateCreated |
2009-5-1
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| pubmed:databankReference | |
| pubmed:abstractText |
A strictly anaerobic, mesophilic, cellulolytic bacterial strain, designated CDT-1(T), was isolated from rice-straw residue from a methanogenic reactor treating waste from cattle farms. The isolation was performed using enrichment culture with filter paper as a substrate. Cells stained Gram-negative, but reacted Gram-positively in the KOH test. Cells were slightly curved rods and were motile by means of peritrichous flagella. The strain produced yellow pigment when grown on filter-paper fragments. Although spore formation was not confirmed microscopically, thermotolerant cells were produced when the strain was grown on filter paper. The optimum temperature for growth was 33 degrees C and the optimum pH was 7.4. Oxidase, catalase and nitrate-reducing activities were absent. The strain utilized xylose, fructose, glucose, cellobiose, xylooligosaccharide, cellulose (filter-paper fragments and ball-milled filter paper) and xylan. The major fermentation products were acetate, ethanol, H(2) and CO(2). The major cellular fatty acids were iso-C(15 : 0), iso-C(14 : 0) and C(16 : 0) DMA. The cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid. The genomic DNA G+C content was 40.7 mol%. On the basis of 16S rRNA gene sequence similarities, strain CDT-1(T) could be placed in cluster III of the genus Clostridium, being closely related to type strains of Clostridium hungatei (96.6 % sequence similarity), Clostridium termitidis (96.2 %) and Clostridium papyrosolvens (96.1 %). On the basis of the cellular, physiological and phylogenetic differences between CDT-1(T) and its close relatives, this strain represents a novel species of the genus Clostridium, for which the name Clostridium sufflavum sp. nov. is proposed. The type strain is CDT-1(T) (=JCM 14807(T)=DSM 19573(T)).
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
May
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| pubmed:issn |
1466-5026
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
59
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
981-6
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| pubmed:meshHeading |
pubmed-meshheading:19406778-Anaerobiosis,
pubmed-meshheading:19406778-Animal Husbandry,
pubmed-meshheading:19406778-Animals,
pubmed-meshheading:19406778-Bacterial Typing Techniques,
pubmed-meshheading:19406778-Base Composition,
pubmed-meshheading:19406778-Bioreactors,
pubmed-meshheading:19406778-Cattle,
pubmed-meshheading:19406778-Clostridium,
pubmed-meshheading:19406778-Fatty Acids,
pubmed-meshheading:19406778-Genes, rRNA,
pubmed-meshheading:19406778-Japan,
pubmed-meshheading:19406778-Methane,
pubmed-meshheading:19406778-Molecular Sequence Data,
pubmed-meshheading:19406778-Oryza sativa,
pubmed-meshheading:19406778-Phylogeny,
pubmed-meshheading:19406778-RNA, Ribosomal, 16S,
pubmed-meshheading:19406778-Refuse Disposal,
pubmed-meshheading:19406778-Sequence Analysis, DNA,
pubmed-meshheading:19406778-Species Specificity
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| pubmed:year |
2009
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| pubmed:articleTitle |
Clostridium sufflavum sp. nov., isolated from a methanogenic reactor treating cattle waste.
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| pubmed:affiliation |
Faculty of Agriculture, Yamagata University, Wakaba-machi 1-23, Tsuruoka, Yamagata 997-8555, Japan.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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