Linked Life Data

19393604

Source:http://linkedlifedata.com/resource/pubmed/id/19393604

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2009-5-21
pubmed:abstractText
The Fcalpha/mu receptor (Fcalpha/microR), a type I transmembrane protein, is an immunoglobulin Fc receptor for both IgA and IgM. Its functions in immune defense are not clear at present. In this work, human Fcalpha/microR was expressed in CHO, 293T, and COS-7 cells to study its biochemical functions. Fcalpha/microR expressed by CHO and 293T was only in monomer form in cytoplasma and the monomeric receptor could not bind IgA or IgM. In comparison, Fcalpha/microR expressed by COS-7 cells had both monomer and dimer forms. The binding assay showed that Fcalpha/microR expressed by COS-7 cells could bind IgM strongly and IgA weakly, implying that dimeric receptor could be expressed on cell membrane and functioned. The bound IgM could be internalized and the internalization was abolished when the cytoplasmic domain of Fcalpha/microR was truncated. Therefore, the cytoplasmic portion of human Fcalpha/microR is required in the internalization.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1090-2163
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
258
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
78-82
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Cytoplasmic domain of human Fcalpha/mu receptor is required for ligand internalization.
pubmed:affiliation
Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't