1939151

Source:http://linkedlifedata.com/resource/pubmed/id/1939151

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Predicate	Object
rdf:type	pubmed:Citation
lifeskim:mentions	umls-concept:C0006100, umls-concept:C0010453, umls-concept:C0031669, umls-concept:C0033634, umls-concept:C0227651, umls-concept:C0598312, umls-concept:C1280500
pubmed:issue	31
pubmed:dateCreated	1991-12-13
pubmed:abstractText	The effects of the neuropeptide bradykinin (BK) and its natural proteolytic fragment Des-Arg9 bradykinin (DBK) on DNA synthesis and phospholipase C activation were investigated in cultured mesangial cells. DBK, acting through a distinct bradykinin receptor, induced DNA synthesis in serum-starved cultured mesangial cells. The effect of DBK was dose dependent (ED50 = 0.6 microM) and was strongly potentiated by insulin. Under the same conditions, BK had no effect. Down-regulation of protein kinase C by long term pretreatment with 12-O-tetradecanoylphorbol-13-acetate (TPA) markedly reduced DBK-induced DNA synthesis. In the same way, co-incubation with the protein kinase C inhibitor staurosporine potently attenuated the response to DBK, suggesting a role of protein kinase C in DBK-induced mitogenesis. Analysis of phosphoproteins from 32P-labeled mesangial cells by two-dimensional gel electrophoresis revealed that DBK, like TPA but not BK, induced a net increase in the phosphorylation of an acidic cellular protein migrating with an apparent Mr = 80,000 (termed 80K), identified as a major and specific substrate of protein kinase C. Phosphorylation of the 80K protein by DBK or TPA was completely abolished in cells depleted of protein kinase C. DBK and TPA also induced an increase in phosphorylation of an Mr = 28,000 protein. Moreover, DBK but not TPA stimulated the phosphorylation of an Mr = 18,000 protein in normal as well as in protein kinase C-depleted cells. Analysis of phospholipase C activation revealed that DBK induced a large and sustained increase in diacylglycerol production and inositol phosphate accumulation over a 10-min incubation. BK had only a minor effect on both parameters. These results demonstrate that DBK, but not BK, modulates DNA synthesis through protein kinase C activation in cultured mesangial cells.
pubmed:language	eng
pubmed:journal	http://linkedlifedata.com/resource/pubmed/journal/2985121R
pubmed:citationSubset	IM
pubmed:chemical	http://linkedlifedata.com/resource/pubmed/chemical/Alkaloids, http://linkedlifedata.com/resource/pubmed/chemical/Bradykinin, http://linkedlifedata.com/resource/pubmed/chemical/DNA, http://linkedlifedata.com/resource/pubmed/chemical/Diglycerides, http://linkedlifedata.com/resource/pubmed/chemical/Inositol Phosphates, http://linkedlifedata.com/resource/pubmed/chemical/Mitogens, http://linkedlifedata.com/resource/pubmed/chemical/Phosphoproteins, http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C, http://linkedlifedata.com/resource/pubmed/chemical/Staurosporine, http://linkedlifedata.com/resource/pubmed/chemical/Tetradecanoylphorbol Acetate, http://linkedlifedata.com/resource/pubmed/chemical/Type C Phospholipases, http://linkedlifedata.com/resource/pubmed/chemical/bradykinin, des-Arg(9)-
pubmed:status	MEDLINE
pubmed:month	Nov
pubmed:issn	0021-9258
pubmed:author	pubmed-author:DarbonJ MJM, pubmed-author:IssandouMM
pubmed:issnType	Print
pubmed:day	5
pubmed:volume	266
pubmed:owner	NLM
pubmed:authorsComplete	Y
pubmed:pagination	21037-43
pubmed:dateRevised	2007-11-15
pubmed:meshHeading	pubmed-meshheading:1939151-Alkaloids, pubmed-meshheading:1939151-Animals, pubmed-meshheading:1939151-Bradykinin, pubmed-meshheading:1939151-Cell Division, pubmed-meshheading:1939151-Cells, Cultured, pubmed-meshheading:1939151-DNA, pubmed-meshheading:1939151-Diglycerides, pubmed-meshheading:1939151-Dose-Response Relationship, Drug, pubmed-meshheading:1939151-Electrophoresis, Gel, Two-Dimensional, pubmed-meshheading:1939151-Enzyme Activation, pubmed-meshheading:1939151-Glomerular Mesangium, pubmed-meshheading:1939151-Inositol Phosphates, pubmed-meshheading:1939151-Mitogens, pubmed-meshheading:1939151-Phosphoproteins, pubmed-meshheading:1939151-Protein Kinase C, pubmed-meshheading:1939151-Rats, pubmed-meshheading:1939151-Staurosporine, pubmed-meshheading:1939151-Structure-Activity Relationship, pubmed-meshheading:1939151-Tetradecanoylphorbol Acetate, pubmed-meshheading:1939151-Type C Phospholipases
pubmed:year	1991
pubmed:articleTitle	Des-Arg9 bradykinin modulates DNA synthesis, phospholipase C, and protein kinase C in cultured mesangial cells. Distinction from effects of bradykinin.
pubmed:affiliation	Institut National de la Santé et de la Recherche Médicale, Faculté de médecine, Toulouse, France.
pubmed:publicationType	Journal Article, In Vitro, Research Support, Non-U.S. Gov't