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rdf:type |
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lifeskim:mentions |
umls-concept:C0043240,
umls-concept:C0205132,
umls-concept:C0314603,
umls-concept:C0332120,
umls-concept:C0374711,
umls-concept:C1314939,
umls-concept:C1514925,
umls-concept:C1517938,
umls-concept:C1704259,
umls-concept:C1705181,
umls-concept:C1705987
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pubmed:issue |
4
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pubmed:dateCreated |
2009-4-24
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pubmed:abstractText |
Long interspersed elements (LINEs) are transposable elements that proliferate within eukaryotic genomes, having a large impact on eukaryotic genome evolution. LINEs mobilize via a process called retrotransposition. Although the role of the LINE-encoded protein(s) in retrotransposition has been extensively investigated, the participation of host-encoded factors in retrotransposition remains unclear. To address this issue, we examined retrotransposition frequencies of two structurally different LINEs--zebrafish ZfL2-2 and human L1--in knockout chicken DT40 cell lines deficient in genes involved in the non-homologous end-joining (NHEJ) repair of DNA and in human HeLa cells treated with a drug that inhibits NHEJ. Deficiencies of NHEJ proteins decreased retrotransposition frequencies of both LINEs in these cells, suggesting that NHEJ is involved in LINE retrotransposition. More precise characterization of ZfL2-2 insertions in DT40 cells permitted us to consider the possibility of dual roles for NHEJ in LINE retrotransposition, namely to ensure efficient integration of LINEs and to restrict their full-length formation.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/19390601-10331276,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/19390601-9809069
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
1553-7404
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
5
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
e1000461
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:19390601-Animals,
pubmed-meshheading:19390601-Chickens,
pubmed-meshheading:19390601-DNA Repair,
pubmed-meshheading:19390601-DNA Repair Enzymes,
pubmed-meshheading:19390601-HeLa Cells,
pubmed-meshheading:19390601-Humans,
pubmed-meshheading:19390601-Long Interspersed Nucleotide Elements,
pubmed-meshheading:19390601-Models, Genetic,
pubmed-meshheading:19390601-Mutagenesis, Insertional,
pubmed-meshheading:19390601-Zebrafish
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pubmed:year |
2009
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pubmed:articleTitle |
Genetic evidence that the non-homologous end-joining repair pathway is involved in LINE retrotransposition.
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pubmed:affiliation |
Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Midori-ku, Yokohama, Kanagawa, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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