Linked Life Data

19386353

Source:http://linkedlifedata.com/resource/pubmed/id/19386353

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
2009-8-17
pubmed:abstractText
There is limited information regarding the process of tissue remodeling in fibroblastic foci associated with idiopathic pulmonary fibrosis. The aim of this study was to identify the different pathologic stages of tissue remodeling in fibroblastic foci based on the histopathologic differences in the glycosaminoglycan distribution and collagen deposition. In addition, we also aimed at clarifying the stage-specific characteristics by taking into consideration the expression pattern of matrix metalloproteinase and angiogenesis. Lung biopsies of 16 patients with idiopathic pulmonary fibrosis were used. The presence of glycosaminoglycans was detected by Alcian blue staining, and type I collagen was detected by immunohistochemical analysis with a primary antibody specific to the cross-linked carboxyterminal telopeptide of type I collagen. The fibroblastic foci characterized by the expression intensity of Alcian blue and telopeptide of type I collagen were divided into 3 groups, namely, Alcian blue(+)telopeptide of type I collagen(weak), Alcian blue(+)telopeptide of type I collagen(+), and Alcian blue(weak)telopeptide of type I collagen(+); consequently, 3 new stages were defined--stages I, II, and III, respectively. A significant inverse correlation was observed between the area densities of Alcian blue(+) and telopeptide of type I collagen(+) in fibroblastic foci. Stage I was characterized by the expression of matrix metalloproteinase-2 and tissue inhibitor of matrix metalloprotease-2 in fibroblasts and the overlying epithelium of fibroblastic foci, and also the absence of capillary angiogenesis. In contrast, the expression of these proteins was attenuated in stage III, except for that of matrix metalloproteinase-2 in fibroblasts. In stages II and III, capillary angiogenesis was observed. Lymphangiogenesis was undetected in all the 3 stages. Thus, pathologic staging helps understand the roles of the factors involved in tissue remodeling in idiopathic pulmonary fibrosis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
1532-8392
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
40
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1278-87
pubmed:meshHeading
pubmed-meshheading:19386353-Aged, pubmed-meshheading:19386353-Alcian Blue, pubmed-meshheading:19386353-Case-Control Studies, pubmed-meshheading:19386353-Collagen Type I, pubmed-meshheading:19386353-Coloring Agents, pubmed-meshheading:19386353-Extracellular Matrix, pubmed-meshheading:19386353-Female, pubmed-meshheading:19386353-Fibroblasts, pubmed-meshheading:19386353-Fibrosis, pubmed-meshheading:19386353-Glycosaminoglycans, pubmed-meshheading:19386353-Histocytochemistry, pubmed-meshheading:19386353-Humans, pubmed-meshheading:19386353-Idiopathic Pulmonary Fibrosis, pubmed-meshheading:19386353-Immunohistochemistry, pubmed-meshheading:19386353-Male, pubmed-meshheading:19386353-Matrix Metalloproteinase 2, pubmed-meshheading:19386353-Middle Aged, pubmed-meshheading:19386353-Tissue Inhibitor of Metalloproteinase-2
pubmed:year
2009
pubmed:articleTitle
The definition of fibrogenic processes in fibroblastic foci of idiopathic pulmonary fibrosis based on morphometric quantification of extracellular matrices.
pubmed:affiliation
Department of Pathology, Tohoku University Graduate School of Medicine, Sendai, Miyagi 980-8575, Japan. yamam@mail.tains.tohoku.ac.jp
pubmed:publicationType
Journal Article