Linked Life Data

19384744

Source:http://linkedlifedata.com/resource/pubmed/id/19384744

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2010-5-27
pubmed:abstractText
The use of prometaphase chromosomes prepared for high-resolution imaging is essential for accurate cytogenetic investigations. The process of Giemsa chromosome banding (G-banding), however, is often time consuming and difficult to standardize owing to differing ambient conditions and inter-operator variability. Consequently, many laboratories currently are introducing automatic metaphase finder and analysis systems to achieve the goals of higher throughput of samples and more consistent chromosome quality. In this context, we investigated the use of automation to improve the G-banding process. We investigated the use of the Shandon Thermo Varistain Gemini automatic stainer to replicate the manual process of G-banding. We compared the current manual method and the automated procedure and found that automation provided equivalent quality, and increased consistency while decreasing the time required and reducing the cost per preparation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1473-7760
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
84
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
337-45
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Automating Giemsa banding of chromosomes: protocol for and evaluation of the use of a programmable, high-throughput automatic stainer.
pubmed:affiliation
South East Scotland Cytogenetics Service, Western General Hospital, Edinburgh EH4 2XU, Scotland, UK. Charlotte.Gregory@luht.scot.nhs.uk
pubmed:publicationType
Journal Article