| pubmed-article:19383686 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19383686 | lifeskim:mentions | umls-concept:C0014834 | lld:lifeskim |
| pubmed-article:19383686 | lifeskim:mentions | umls-concept:C0019878 | lld:lifeskim |
| pubmed-article:19383686 | lifeskim:mentions | umls-concept:C0021467 | lld:lifeskim |
| pubmed-article:19383686 | lifeskim:mentions | umls-concept:C0018270 | lld:lifeskim |
| pubmed-article:19383686 | lifeskim:mentions | umls-concept:C0868955 | lld:lifeskim |
| pubmed-article:19383686 | lifeskim:mentions | umls-concept:C0021469 | lld:lifeskim |
| pubmed-article:19383686 | pubmed:issue | Pt 6 | lld:pubmed |
| pubmed-article:19383686 | pubmed:dateCreated | 2009-6-2 | lld:pubmed |
| pubmed-article:19383686 | pubmed:abstractText | In Escherichia coli homocysteine (Hcy) is metabolically converted to the thioester Hcy-thiolactone in ATP-consuming reactions catalysed by methionyl-, isoleucyl- and leucyl-tRNA synthetases. Here we show that growth inhibition caused by supplementation of E. coli cultures with Hcy is accompanied by greatly increased accumulation of Hcy-thiolactone. Energy dissipation for Hcy editing increases 100-fold in the presence of exogenous Hcy and reaches one mole of ATP unproductively dissipated for Hcy-thiolactone synthesis per each mole of ATP that is consumed for methionine activation. Inhibiting Hcy-thiolactone synthesis with isoleucine, leucine or methionine accelerates bacterial growth in Hcy-supplemented cultures. Growth rates in Hcy-inhibited cultures are inversely related to the accumulation of Hcy-thiolactone. We also show that the levels of protein N-linked Hcy modestly increase in E. coli cells in Hcy-supplemented cultures. The results suggest that Hcy editing restrains bacterial growth. | lld:pubmed |
| pubmed-article:19383686 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19383686 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19383686 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:19383686 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19383686 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19383686 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19383686 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19383686 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19383686 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19383686 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19383686 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19383686 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19383686 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:19383686 | pubmed:issn | 1350-0872 | lld:pubmed |
| pubmed-article:19383686 | pubmed:author | pubmed-author:JakubowskiHie... | lld:pubmed |
| pubmed-article:19383686 | pubmed:author | pubmed-author:SikoraMartaM | lld:pubmed |
| pubmed-article:19383686 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:19383686 | pubmed:volume | 155 | lld:pubmed |
| pubmed-article:19383686 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19383686 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19383686 | pubmed:pagination | 1858-65 | lld:pubmed |
| pubmed-article:19383686 | pubmed:meshHeading | pubmed-meshheading:19383686... | lld:pubmed |
| pubmed-article:19383686 | pubmed:meshHeading | pubmed-meshheading:19383686... | lld:pubmed |
| pubmed-article:19383686 | pubmed:meshHeading | pubmed-meshheading:19383686... | lld:pubmed |
| pubmed-article:19383686 | pubmed:meshHeading | pubmed-meshheading:19383686... | lld:pubmed |
| pubmed-article:19383686 | pubmed:meshHeading | pubmed-meshheading:19383686... | lld:pubmed |
| pubmed-article:19383686 | pubmed:meshHeading | pubmed-meshheading:19383686... | lld:pubmed |
| pubmed-article:19383686 | pubmed:meshHeading | pubmed-meshheading:19383686... | lld:pubmed |
| pubmed-article:19383686 | pubmed:meshHeading | pubmed-meshheading:19383686... | lld:pubmed |
| pubmed-article:19383686 | pubmed:meshHeading | pubmed-meshheading:19383686... | lld:pubmed |
| pubmed-article:19383686 | pubmed:meshHeading | pubmed-meshheading:19383686... | lld:pubmed |
| pubmed-article:19383686 | pubmed:year | 2009 | lld:pubmed |
| pubmed-article:19383686 | pubmed:articleTitle | Homocysteine editing and growth inhibition in Escherichia coli. | lld:pubmed |
| pubmed-article:19383686 | pubmed:affiliation | Department of Microbiology and Molecular Genetics, UMDNJ-New Jersey Medical School, International Center for Public Health, Newark, NJ 07101, USA. | lld:pubmed |
| pubmed-article:19383686 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:19383686 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| entrez-gene:946643 | entrezgene:pubmed | pubmed-article:19383686 | lld:entrezgene |
| entrez-gene:944761 | entrezgene:pubmed | pubmed-article:19383686 | lld:entrezgene |
| entrez-gene:947497 | entrezgene:pubmed | pubmed-article:19383686 | lld:entrezgene |
