Source:http://linkedlifedata.com/resource/pubmed/id/19383686
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
Pt 6
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pubmed:dateCreated |
2009-6-2
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pubmed:abstractText |
In Escherichia coli homocysteine (Hcy) is metabolically converted to the thioester Hcy-thiolactone in ATP-consuming reactions catalysed by methionyl-, isoleucyl- and leucyl-tRNA synthetases. Here we show that growth inhibition caused by supplementation of E. coli cultures with Hcy is accompanied by greatly increased accumulation of Hcy-thiolactone. Energy dissipation for Hcy editing increases 100-fold in the presence of exogenous Hcy and reaches one mole of ATP unproductively dissipated for Hcy-thiolactone synthesis per each mole of ATP that is consumed for methionine activation. Inhibiting Hcy-thiolactone synthesis with isoleucine, leucine or methionine accelerates bacterial growth in Hcy-supplemented cultures. Growth rates in Hcy-inhibited cultures are inversely related to the accumulation of Hcy-thiolactone. We also show that the levels of protein N-linked Hcy modestly increase in E. coli cells in Hcy-supplemented cultures. The results suggest that Hcy editing restrains bacterial growth.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Triphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Amino Acyl-tRNA Synthetases,
http://linkedlifedata.com/resource/pubmed/chemical/Homocysteine,
http://linkedlifedata.com/resource/pubmed/chemical/Isoleucine,
http://linkedlifedata.com/resource/pubmed/chemical/Leucine,
http://linkedlifedata.com/resource/pubmed/chemical/Methionine,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium Acetate,
http://linkedlifedata.com/resource/pubmed/chemical/homocysteine thiolactone
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
1350-0872
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
155
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1858-65
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pubmed:meshHeading |
pubmed-meshheading:19383686-Adenosine Triphosphate,
pubmed-meshheading:19383686-Amino Acyl-tRNA Synthetases,
pubmed-meshheading:19383686-Energy Metabolism,
pubmed-meshheading:19383686-Escherichia coli,
pubmed-meshheading:19383686-Homocysteine,
pubmed-meshheading:19383686-Isoleucine,
pubmed-meshheading:19383686-Leucine,
pubmed-meshheading:19383686-Methionine,
pubmed-meshheading:19383686-Protein Biosynthesis,
pubmed-meshheading:19383686-Sodium Acetate
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pubmed:year |
2009
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pubmed:articleTitle |
Homocysteine editing and growth inhibition in Escherichia coli.
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pubmed:affiliation |
Department of Microbiology and Molecular Genetics, UMDNJ-New Jersey Medical School, International Center for Public Health, Newark, NJ 07101, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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