Linked Life Data

19381593

Source:http://linkedlifedata.com/resource/pubmed/id/19381593

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2009-4-21
pubmed:abstractText
Silver staining detects proteins after electrophoretic separation on polyacrylamide gels. Its main positive features are its excellent sensitivity (in the low nanogram range) and the use of very simple and cheap equipment and chemicals. The sequential phases of silver staining are protein fixation, then sensitization, then silver impregnation, and finally image development. Several variants of silver staining are described here, which can be completed in a time range from 2 h to 1 day after the end of the electrophoretic separation. Once completed, the stain is stable for several weeks.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1064-3745
pubmed:author
pubmed:issnType
Print
pubmed:volume
519
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
339-50
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Silver staining of proteins in 2DE gels.
pubmed:affiliation
CEA-DSV-iRTSV/LBBSI and UMR CNRS 5092, CEA Grenoble, 17 rue des martyrs, F-38054 Grenoble Cedex 9, France.
pubmed:publicationType
Journal Article