Linked Life Data

19378182

Source:http://linkedlifedata.com/resource/pubmed/id/19378182

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2009-4-20
pubmed:abstractText
Protein-DNA interactions underpin life and play key roles in all cellular processes and functions including DNA transcription, packaging, replication, and repair. Identifying and examining the nature of these interactions is therefore a crucial prerequisite to understand the molecular basis of how these fundamental processes take place. The application of fluorescence techniques and in particular fluorescence resonance energy transfer (FRET) to provide structural and kinetic information has experienced a stunning growth during the past decade. This has been mostly promoted by new advances in the preparation of dye-labeled nucleic acids and proteins and in optical sensitivity, where its implementation at the level of individual molecules has opened a new biophysical frontier. Nowadays, the application of FRET-based techniques to the analysis of protein-DNA interactions spans from the classical steady-state and time-resolved methods averaging over large ensembles to the analysis of distances, conformational changes, and enzymatic reactions in individual Protein-DNA complexes. This chapter introduces the practical aspects of applying these methods for the study of Protein-DNA interactions.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1064-3745
pubmed:author
pubmed:issnType
Print
pubmed:volume
543
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
475-502
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Functional studies of DNA-protein interactions using FRET techniques.
pubmed:affiliation
Département de Biologie, Faculté des Sciences, Université de Sherbrooke, 2500 boul. Université, Sherbrooke, QC, Canada, J1K 2R1.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't