Linked Life Data

19378170

Source:http://linkedlifedata.com/resource/pubmed/id/19378170

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2009-4-20
pubmed:abstractText
The ensemble of the genes in the mammalian genome is organized into a structure of DNA and proteins known as chromatin. The control of gene expression by the proteins that bind to chromatin regulates many cell processes, such as differentiation and proliferation. Transcription of protein-encoding genes in mammalian cells is performed by the concerted action of the RNA polymerase II holoenzyme, transcription factors, co-activator complexes that bind to the promoter areas of genes. In addition, different proteins can interact with these complexes and chromatin to create a repressive state. In order to fundamentally understand transcriptional control, it is important to define the areas that these proteins will bind. Classical laboratory techniques unable to provide distinct locations of these factors have now been replaced by the chromatin immunoprecipitation (ChIP) assay. The ChIP technique allows us to isolate chromatin along with its associated proteins from cells and analyse the binding sites of specific proteins and complexes at high resolution.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1064-3745
pubmed:author
pubmed:issnType
Print
pubmed:volume
543
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
243-51
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Chromatin immunoprecipitation in mammalian cells.
pubmed:affiliation
Département de Biologie, Faculté des Sciences, Université de Sherbrooke, 2500 Boulevard de l'Université, Sherbrooke, QC, Canada J1K 2R1.
pubmed:publicationType
Journal Article