Linked Life Data

19378105

Source:http://linkedlifedata.com/resource/pubmed/id/19378105

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2009-4-20
pubmed:abstractText
The availability of microarray technology for zebrafish research has enabled the expression of tens of thousands of genes to be studied simultaneously in one experiment. The experiment usually involves measuring and comparing the relative abundance of tens of thousands of mRNA species in experimental samples obtained from mutant versus wild-type embryos, disease versus normal tissues, embryos/fish of different developmental stages, physiologic states, or from multiple treatments and/or time-points. A microarray experiment comprised of several stages can be divided into two distinct parts (i.e., the "wet-lab" and the "dry-lab"). The success of a microarray experiment hinges on the "wet-lab" procedures, which include technology that allows for generation of arrays with very high-density DNA where tens of thousands of genes are represented in an area smaller than a standard glass microscope slide, and procedures that enable extraction of high-quality RNA, efficient fluorescent labeling of nucleic acids, as well as specific hybridization of fluorescent labeled-samples with arrayed probes. This chapter describes these "wet-lab" procedures. "Dry-lab" procedures are described in the next chapter.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1064-3745
pubmed:author
pubmed:issnType
Print
pubmed:volume
546
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
175-95
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Zebrafish spotted-microarray for genome-wide expression profiling experiments. Part I: array printing and hybridization.
pubmed:affiliation
Department of Biological Sciences, S3-Level 5, 14 Science Drive 4, National University of Singapore, Singapore, 117543.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't