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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
2009-4-20
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pubmed:abstractText |
This chapter describes a method for generation of the high-titer pseudotyped Moloney murine leukemia virus (MLV) that efficiently infects zebrafish embryos (i.e., more than 25 retroviral copies per cell). Injection techniques are also described for production of the retrovirus-infected mosaic "founder" fish. We describe a quantitative PCR (qPCR)-based assay as a quick way to assess the infectivity after each round of viral production and injection. Most of the required equipment is commercially available and commonly present in most research laboratories.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1064-3745
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
546
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
13-30
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pubmed:meshHeading |
pubmed-meshheading:19378095-Animals,
pubmed-meshheading:19378095-Animals, Genetically Modified,
pubmed-meshheading:19378095-Cell Line,
pubmed-meshheading:19378095-Chimerism,
pubmed-meshheading:19378095-DNA,
pubmed-meshheading:19378095-Female,
pubmed-meshheading:19378095-Gene Transfer Techniques,
pubmed-meshheading:19378095-Genome,
pubmed-meshheading:19378095-Humans,
pubmed-meshheading:19378095-Male,
pubmed-meshheading:19378095-Membrane Glycoproteins,
pubmed-meshheading:19378095-Moloney murine leukemia virus,
pubmed-meshheading:19378095-Mutagenesis, Insertional,
pubmed-meshheading:19378095-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:19378095-Viral Envelope Proteins,
pubmed-meshheading:19378095-Zebrafish
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pubmed:year |
2009
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pubmed:articleTitle |
Production of pseudotyped retrovirus and the generation of proviral transgenic zebrafish.
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pubmed:affiliation |
Cell and Developmental Biology, Vanderbilt University, TN, Nashville, USA.
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Intramural
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