Linked Life Data

19378089

Source:http://linkedlifedata.com/resource/pubmed/id/19378089

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2009-4-20
pubmed:abstractText
In 1988, two separate investigators reported a novel method of detecting unstained protein bands on polyvinylidene fluoride (PVDF) membranes using white light transillumination. This simple method exploits the intrinsic hydrophobicity of PVDF membrane, which enables the visual observation of transferred protein bands due to differential wetting patterns between protein bands and the membrane itself. This method applies only to hydrophobic PVDF membranes, because hydrophilic membranes such as nitrocellulose wet out completely, rendering the protein bands invisible by transillumination. Transilluminational protein visualization can detect submicrogram quantities of proteins while circumventing the use of protein stains, which can potentially interfere with downstream analysis such as N-terminal sequencing. In this chapter, we demonstrate efficient transilluminational protein visualization on a recently introduced low-fluorescence PVDF membrane, normally used for downstream fluorescent immunodetection.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1064-3745
pubmed:author
pubmed:issnType
Print
pubmed:volume
536
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
527-31
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Visualization of unstained protein bands on PVDF.
pubmed:affiliation
Millipore Corporation, 17 Cherry Hill Drive, Danvers, MA, 01923, USA.
pubmed:publicationType
Journal Article