Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2009-6-29
pubmed:abstractText
A major limitation to the application of stem-cell therapy to repair ischemic heart damage is the low survival of transplanted cells in the heart, possibly due to poor oxygenation. We hypothesized that hyperbaric oxygenation (HBO) can be used as an adjuvant treatment to augment stem-cell therapy. Therefore, the goal of this study was to evaluate the effect of HBO on the engraftment of rat bone marrow-derived mesenchymal stem cells (MSCs) transplanted in infarct rat hearts. Myocardial infarction (MI) was induced in Fisher-344 rats by permanently ligating the left-anterior-descending coronary artery. MSCs, labeled with fluorescent superparamagnetic iron oxide (SPIO) particles, were transplanted in the infarct and peri-infarct regions of the MI hearts. HBO (100% oxygen at 2 ATA for 90 min) was administered daily for 2 weeks. Four MI groups were used: untreated (MI); HBO; MSC; MSC+HBO. Echocardiography, electro-vectorcardiography, and magnetic resonance imaging were used for functional evaluations. The engraftment of transplanted MSCs in the heart was confirmed by SPIO fluorescence and Prussian-blue staining. Immunohistochemical staining was used to identify key cellular and molecular markers including CD29, troponin-T, connexin-43, VEGF, alpha-smooth-muscle actin, and von Willebrand factor in the tissue. Compared to MI and MSC groups, the MSC+HBO group showed a significantly increased recovery of cardiac function including left-ventricular (LV) ejection fraction, fraction shortening, LV wall thickness, and QRS vector. Further, HBO treatment significantly increased the engraftment of CD29-positive cells, expression of connexin-43, troponin-T and VEGF, and angiogenesis in the infarct tissue. Thus, HBO appears to be a potential and clinically-viable adjuvant treatment for myocardial stem-cell therapy.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/19376124-10074487, http://linkedlifedata.com/resource/pubmed/commentcorrection/19376124-11263675, http://linkedlifedata.com/resource/pubmed/commentcorrection/19376124-11287958, http://linkedlifedata.com/resource/pubmed/commentcorrection/19376124-12068011, http://linkedlifedata.com/resource/pubmed/commentcorrection/19376124-12078791, http://linkedlifedata.com/resource/pubmed/commentcorrection/19376124-12537217, http://linkedlifedata.com/resource/pubmed/commentcorrection/19376124-12778165, http://linkedlifedata.com/resource/pubmed/commentcorrection/19376124-12910262, http://linkedlifedata.com/resource/pubmed/commentcorrection/19376124-14399296, http://linkedlifedata.com/resource/pubmed/commentcorrection/19376124-15459609, http://linkedlifedata.com/resource/pubmed/commentcorrection/19376124-16286608, http://linkedlifedata.com/resource/pubmed/commentcorrection/19376124-16299259, http://linkedlifedata.com/resource/pubmed/commentcorrection/19376124-16513203, http://linkedlifedata.com/resource/pubmed/commentcorrection/19376124-16741354, http://linkedlifedata.com/resource/pubmed/commentcorrection/19376124-16930572, http://linkedlifedata.com/resource/pubmed/commentcorrection/19376124-16945821, http://linkedlifedata.com/resource/pubmed/commentcorrection/19376124-17142337, http://linkedlifedata.com/resource/pubmed/commentcorrection/19376124-17230207, http://linkedlifedata.com/resource/pubmed/commentcorrection/19376124-17478584, http://linkedlifedata.com/resource/pubmed/commentcorrection/19376124-17660391, http://linkedlifedata.com/resource/pubmed/commentcorrection/19376124-17721512, http://linkedlifedata.com/resource/pubmed/commentcorrection/19376124-17951319, http://linkedlifedata.com/resource/pubmed/commentcorrection/19376124-18006467, http://linkedlifedata.com/resource/pubmed/commentcorrection/19376124-18290313, http://linkedlifedata.com/resource/pubmed/commentcorrection/19376124-18466917, http://linkedlifedata.com/resource/pubmed/commentcorrection/19376124-18471250, http://linkedlifedata.com/resource/pubmed/commentcorrection/19376124-18687994, http://linkedlifedata.com/resource/pubmed/commentcorrection/19376124-18703748, http://linkedlifedata.com/resource/pubmed/commentcorrection/19376124-18948617, http://linkedlifedata.com/resource/pubmed/commentcorrection/19376124-8403338, http://linkedlifedata.com/resource/pubmed/commentcorrection/19376124-8628361, http://linkedlifedata.com/resource/pubmed/commentcorrection/19376124-9778551
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1095-8584
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
47
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
275-87
pubmed:dateRevised
2011-6-8
pubmed:meshHeading
pubmed-meshheading:19376124-Action Potentials, pubmed-meshheading:19376124-Animals, pubmed-meshheading:19376124-Antigens, CD29, pubmed-meshheading:19376124-Blood Vessels, pubmed-meshheading:19376124-Connexin 43, pubmed-meshheading:19376124-Ferric Compounds, pubmed-meshheading:19376124-Fibrosis, pubmed-meshheading:19376124-Hyperbaric Oxygenation, pubmed-meshheading:19376124-Mesenchymal Stem Cell Transplantation, pubmed-meshheading:19376124-Myocardial Infarction, pubmed-meshheading:19376124-Myocardium, pubmed-meshheading:19376124-Neovascularization, Physiologic, pubmed-meshheading:19376124-Partial Pressure, pubmed-meshheading:19376124-Rats, pubmed-meshheading:19376124-Rats, Inbred F344, pubmed-meshheading:19376124-Recovery of Function, pubmed-meshheading:19376124-Troponin T, pubmed-meshheading:19376124-Vascular Endothelial Growth Factor A, pubmed-meshheading:19376124-Ventricular Function, Left
pubmed:year
2009
pubmed:articleTitle
Hyperbaric oxygenation enhances transplanted cell graft and functional recovery in the infarct heart.
pubmed:affiliation
Davis Heart and Lung Research Institute, Division of Cardiovascular Medicine, Department of Internal Medicine, The Ohio State University, Columbus, OH 43210, USA. mahmood.khan@osumc.edu
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural