Source:http://linkedlifedata.com/resource/pubmed/id/19374886
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:dateCreated |
2009-5-25
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| pubmed:abstractText |
Despite the correlation between changes in the levels of apurinic/apyrimidinic endonuclease and ischemic neuronal damage, no studies have addressed the question of whether increased APE/Ref-1 can prevent ischemic neuronal cell death in vivo. Using an adenoviral vector, we investigated whether increased APE/Ref-1 can inhibit the loss of APE/Ref-1 and thereby prevent oxidative DNA damage after transient focal cerebral ischemia. Mice were subjected to intraluminal suture occlusion of the middle cerebral artery for 1 h, followed by reperfusion. Pre-ischemic treatment of the adenoviral vector was introduced intracerebrally. An adenoviral vector harboring the entire APE/Ref-1 gene sequence or a control virus without the APE/Ref-1 sequence was introduced 3 days before ischemia/reperfusion (I/R). The reduction of APE/Ref-1 occurred before DNA fragmentation, which was shown by temporal and spatial analysis. Increased APE/Ref-1 significantly decreased DNA damage and infarct volume after I/R. In conclusion, increased APE/Ref-1 enhanced DNA repair and inhibited the induction of ischemic oxidative DNA damage and cerebral infarction after I/R.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
1872-6240
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| pubmed:author | |
| pubmed:issnType |
Electronic
|
| pubmed:day |
5
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| pubmed:volume |
1274
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1-10
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| pubmed:meshHeading |
pubmed-meshheading:19374886-Adenoviridae,
pubmed-meshheading:19374886-Animals,
pubmed-meshheading:19374886-Blotting, Western,
pubmed-meshheading:19374886-Cell Death,
pubmed-meshheading:19374886-Cerebrovascular Circulation,
pubmed-meshheading:19374886-DNA Fragmentation,
pubmed-meshheading:19374886-DNA-(Apurinic or Apyrimidinic Site) Lyase,
pubmed-meshheading:19374886-Fluorescent Antibody Technique,
pubmed-meshheading:19374886-Free Radicals,
pubmed-meshheading:19374886-Gene Therapy,
pubmed-meshheading:19374886-Genetic Vectors,
pubmed-meshheading:19374886-In Situ Nick-End Labeling,
pubmed-meshheading:19374886-Infarction, Middle Cerebral Artery,
pubmed-meshheading:19374886-Ischemic Attack, Transient,
pubmed-meshheading:19374886-Laser-Doppler Flowmetry,
pubmed-meshheading:19374886-Male,
pubmed-meshheading:19374886-Mice,
pubmed-meshheading:19374886-Mice, Inbred C57BL,
pubmed-meshheading:19374886-Neurons,
pubmed-meshheading:19374886-Oxidative Stress,
pubmed-meshheading:19374886-Reperfusion Injury
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| pubmed:year |
2009
|
| pubmed:articleTitle |
The adenoviral vector-mediated increase in apurinic/apyrimidinic endonuclease inhibits the induction of neuronal cell death after transient ischemic stroke in mice.
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| pubmed:affiliation |
Department of Neurology and Brain Korea 21 Project for Medical Science, College of Medicine, Yonsei University, Seoul, Republic of Korea.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|