| pubmed-article:19373607 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19373607 | lifeskim:mentions | umls-concept:C0677626 | lld:lifeskim |
| pubmed-article:19373607 | lifeskim:mentions | umls-concept:C0013081 | lld:lifeskim |
| pubmed-article:19373607 | lifeskim:mentions | umls-concept:C1280500 | lld:lifeskim |
| pubmed-article:19373607 | lifeskim:mentions | umls-concept:C1332817 | lld:lifeskim |
| pubmed-article:19373607 | lifeskim:mentions | umls-concept:C0021469 | lld:lifeskim |
| pubmed-article:19373607 | lifeskim:mentions | umls-concept:C0060794 | lld:lifeskim |
| pubmed-article:19373607 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:19373607 | pubmed:dateCreated | 2009-4-17 | lld:pubmed |
| pubmed-article:19373607 | pubmed:abstractText | The aim of this study is to assess whether fucoidan modulates the expression of chemokine ligand 12 (CXCL12)/chemokine receptor 4 (CXCR4) and exerts antitumor activity toward Huh7 hepatoma cells. According to 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays, fucoidan inhibited the growth of Huh7 cells and HepG2 cells in a dose-dependent manner, with a 50% inhibition of cell growth (IC50) of 2.0 and 4.0 mg/ml, respectively. alpha-fetoprotein levels in medium collected from fucoidan-treated cells were significantly decreased in Huh7 cells but not in HepG2 cells. Western blotting revealed that the amount of alpha-fetoprotein was decreased by 1.0 mg/ml of fucoidan in Huh7 cells, whereas it was unchanged in HepG2 cells. In Huh7 cells, CXCL12 mRNA expression was significantly downregulated by 1.0 mg/ml of fucoidan, whereas CXCR4 mRNA expression was unchanged by fucoidan. CXCL12 and CXCR4 mRNA were barely expressed in HepG2 cells. In addition, 1.0 mg/ml of fucoidan mildly arrested the cell cycle and induced apoptosis in Huh7 cells. The findings suggest that fucoidan exhibits antitumor activity toward Huh7 cells through the downregulation of CXCL12 expression. | lld:pubmed |
| pubmed-article:19373607 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19373607 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19373607 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:19373607 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:19373607 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19373607 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19373607 | pubmed:issn | 1532-7914 | lld:pubmed |
| pubmed-article:19373607 | pubmed:author | pubmed-author:NagamineTakea... | lld:pubmed |
| pubmed-article:19373607 | pubmed:author | pubmed-author:KusakabeTakah... | lld:pubmed |
| pubmed-article:19373607 | pubmed:author | pubmed-author:HayakawaKouK | lld:pubmed |
| pubmed-article:19373607 | pubmed:author | pubmed-author:TakadaHisashi... | lld:pubmed |
| pubmed-article:19373607 | pubmed:author | pubmed-author:NakazatoKyoum... | lld:pubmed |
| pubmed-article:19373607 | pubmed:author | pubmed-author:IhaMasahikoM | lld:pubmed |
| pubmed-article:19373607 | pubmed:author | pubmed-author:HisanagaEtsuk... | lld:pubmed |
| pubmed-article:19373607 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:19373607 | pubmed:volume | 61 | lld:pubmed |
| pubmed-article:19373607 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19373607 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19373607 | pubmed:pagination | 340-7 | lld:pubmed |
| pubmed-article:19373607 | pubmed:meshHeading | pubmed-meshheading:19373607... | lld:pubmed |
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| pubmed-article:19373607 | pubmed:meshHeading | pubmed-meshheading:19373607... | lld:pubmed |
| pubmed-article:19373607 | pubmed:year | 2009 | lld:pubmed |
| pubmed-article:19373607 | pubmed:articleTitle | Inhibitory effect of fucoidan on Huh7 hepatoma cells through downregulation of CXCL12. | lld:pubmed |
| pubmed-article:19373607 | pubmed:affiliation | School of Health Science, Gunma University, 3-39-15 Showamachi, Maebashi, Gunma 371-8514, Japan. mine@health.gunma-u.ac.jp | lld:pubmed |
| pubmed-article:19373607 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:19373607 | lld:pubmed |
