| pubmed-article:19372011 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19372011 | lifeskim:mentions | umls-concept:C0014834 | lld:lifeskim |
| pubmed-article:19372011 | lifeskim:mentions | umls-concept:C0015733 | lld:lifeskim |
| pubmed-article:19372011 | lifeskim:mentions | umls-concept:C2347026 | lld:lifeskim |
| pubmed-article:19372011 | lifeskim:mentions | umls-concept:C0370003 | lld:lifeskim |
| pubmed-article:19372011 | lifeskim:mentions | umls-concept:C0033105 | lld:lifeskim |
| pubmed-article:19372011 | lifeskim:mentions | umls-concept:C0370215 | lld:lifeskim |
| pubmed-article:19372011 | lifeskim:mentions | umls-concept:C1554183 | lld:lifeskim |
| pubmed-article:19372011 | pubmed:issue | 3-4 | lld:pubmed |
| pubmed-article:19372011 | pubmed:dateCreated | 2009-9-7 | lld:pubmed |
| pubmed-article:19372011 | pubmed:abstractText | Seventy-six faecal samples were obtained from broilers at slaughterhouse level in Portugal. Samples were inoculated on cefotaxime-supplemented Levine agar plates. Cefotaxime-resistant Escherichia coli isolates were recovered from 32 samples (42.1%), obtaining a total of 34 E. coli isolates (one or two isolates per sample). Susceptibility to 16 antibiotics was studied by disk diffusion method, and 85% of the isolates presented a phenotype of multi-resistance that included antimicrobial agents of at least four different families. Extended-spectrum-beta-lactamases (ESBL) of the TEM and CTX-M groups were detected in 31 ESBL-positive E. coli isolates. Twenty-six isolates harboured the bla(TEM-52) gene and two of them also harboured bla(TEM-1b). The bla(CTX-M-14) gene was identified in three isolates (in association with bla(TEM-1b) in one of them), and bla(CTX-M-32) was demonstrated in two additional isolates. Three of the 34 cefotaxime-resistant isolates (9%) did not produce ESBLs, and two of them presented mutations at positions -42 (C-->T), -18 (G-->A), -1 (C-->T), and +58(C-->T) of the promoter/attenuator region of ampC gene. tet(A) and/or tet(B) genes were detected in all 34 tetracycline-resistant isolates, aadA in all 26 streptomycin-resistant isolates; cmlA in 3 of 6 chloramphenicol-resistant isolates, and aac(3)-II or aac(3)-I + aac(3)-IV genes in all 4 gentamicin-resistant isolates. Different combinations of sul1, sul2 and sul3 genes were demonstrated among the 22 trimethoprim-sulfamethoxazole-resistant isolates. Amino acid changes in GyrA and ParC proteins were identified in all 18 ciprofloxacin-resistant isolates. The results of this study indicate that the intestinal tract of healthy poultry is a reservoir of ESBL-positive E. coli isolates. | lld:pubmed |
| pubmed-article:19372011 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19372011 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19372011 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:19372011 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19372011 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19372011 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19372011 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19372011 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19372011 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:19372011 | pubmed:issn | 1873-2542 | lld:pubmed |
| pubmed-article:19372011 | pubmed:author | pubmed-author:ZarazagaMyria... | lld:pubmed |
| pubmed-article:19372011 | pubmed:author | pubmed-author:TorresCarmenC | lld:pubmed |
| pubmed-article:19372011 | pubmed:author | pubmed-author:MatosManuelaM | lld:pubmed |
| pubmed-article:19372011 | pubmed:author | pubmed-author:SáenzYolandaY | lld:pubmed |
| pubmed-article:19372011 | pubmed:author | pubmed-author:RodriguesJorg... | lld:pubmed |
| pubmed-article:19372011 | pubmed:author | pubmed-author:CostaDanielaD | lld:pubmed |
| pubmed-article:19372011 | pubmed:author | pubmed-author:PoetaPatricia... | lld:pubmed |
| pubmed-article:19372011 | pubmed:author | pubmed-author:VinuéLauraL | lld:pubmed |
| pubmed-article:19372011 | pubmed:author | pubmed-author:CoelhoAna... | lld:pubmed |
| pubmed-article:19372011 | pubmed:author | pubmed-author:SomaloSergioS | lld:pubmed |
| pubmed-article:19372011 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:19372011 | pubmed:day | 18 | lld:pubmed |
| pubmed-article:19372011 | pubmed:volume | 138 | lld:pubmed |
| pubmed-article:19372011 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19372011 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19372011 | pubmed:pagination | 339-44 | lld:pubmed |
| pubmed-article:19372011 | pubmed:meshHeading | pubmed-meshheading:19372011... | lld:pubmed |
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| pubmed-article:19372011 | pubmed:meshHeading | pubmed-meshheading:19372011... | lld:pubmed |
| pubmed-article:19372011 | pubmed:year | 2009 | lld:pubmed |
| pubmed-article:19372011 | pubmed:articleTitle | Prevalence of extended-spectrum beta-lactamase-producing Escherichia coli isolates in faecal samples of broilers. | lld:pubmed |
| pubmed-article:19372011 | pubmed:affiliation | Universidade de Trás-os-Montes e Alto Douro, Departamento de Ciências Veterinárias, Vila Real, Portugal. | lld:pubmed |
| pubmed-article:19372011 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:19372011 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
