rdf:type |
|
lifeskim:mentions |
|
pubmed:issue |
2
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pubmed:dateCreated |
1991-12-13
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pubmed:databankReference |
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pubmed:abstractText |
A cDNA encoding ovine granulocyte-macrophage colony-stimulating factor (GM-CSF) has been cloned using the polymerase chain reaction. The nucleotide sequence is approx. 93% identical to the published bovine GM-CSF-encoding sequence, 84% to the human sequence and 73% to the murine sequence. The deduced amino acid sequence of the ovine GM-CSF protein was found to be 80% identical to both the human and bovine proteins and 57% to the murine protein. Transient expression of recombinant ovine GM-CSF in COS-1 cells was obtained and its biological activity investigated in a bone-marrow colony-forming assay. Ovine GM-CSF was found to promote the formation of granulocyte-macrophage colonies as well as eosinophil, neutrophil and monocyte/macrophage colonies, an activity characteristic of GM-CSF in other species. Recombinant human GM-CSF was found to have no proliferative effect on ovine bone-marrow cells.
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pubmed:language |
eng
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pubmed:journal |
|
pubmed:citationSubset |
IM
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pubmed:chemical |
|
pubmed:status |
MEDLINE
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pubmed:month |
Sep
|
pubmed:issn |
0378-1119
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pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:day |
15
|
pubmed:volume |
105
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
275-9
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:1937025-Amino Acid Sequence,
pubmed-meshheading:1937025-Animals,
pubmed-meshheading:1937025-Base Sequence,
pubmed-meshheading:1937025-Cell Line,
pubmed-meshheading:1937025-Cloning, Molecular,
pubmed-meshheading:1937025-DNA,
pubmed-meshheading:1937025-Granulocyte-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:1937025-Humans,
pubmed-meshheading:1937025-Mice,
pubmed-meshheading:1937025-Molecular Sequence Data,
pubmed-meshheading:1937025-Polymerase Chain Reaction,
pubmed-meshheading:1937025-Sequence Homology, Nucleic Acid,
pubmed-meshheading:1937025-Sheep
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pubmed:year |
1991
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pubmed:articleTitle |
Cloning and expression of a cDNA encoding ovine granulocyte-macrophage colony-stimulating factor.
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pubmed:affiliation |
Moredun Research Institute, Edinburgh, U.K.
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pubmed:publicationType |
Journal Article
|