Linked Life Data

19364659

Source:http://linkedlifedata.com/resource/pubmed/id/19364659

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2009-5-26
pubmed:abstractText
IL-32 is a newly discovered protein found in human and certain primates, but absent in rodent. Various reports suggest its role as a proinflammatory mediator. Since vascular endothelium is critical in inflammation, we investigate IL-32 in endothelial cells. We found that the gene is expressed in human endothelial cells and Akt strongly induces its expression. Sequence analysis indicates IL-32 beta as the major isoform in endothelial cells. Surprisingly, we did not detect any secretion of IL-32 beta in human endothelial cells; instead we observed co-localization of IL-32 beta with endoplasmic reticulum, suggesting IL-32 beta is an intracellular protein in these cells. Promoter analysis identified a minimum required region for IL-32 transcription at -0.1 to +0.5 kb around the initially identified transcription start site. We also defined a transcriptional suppressor-binding site at -2.0 to -1.5 kb. Importantly, RNA ligase mediated rapid amplification of cDNA ends in endothelial cells determined the transcription start site at the 328 bp downstream from the original identified site. Finally, we found a positive correlation of IL-32 levels with human breast cancer and glioblastoma multiforme (GBM). These findings improve our understanding of IL-32 in vascular endothelium. IL-32 expression might be valuable as a biomarker for cancer.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/19364659-12239319, http://linkedlifedata.com/resource/pubmed/commentcorrection/19364659-12477932, http://linkedlifedata.com/resource/pubmed/commentcorrection/19364659-15286730, http://linkedlifedata.com/resource/pubmed/commentcorrection/19364659-15664165, http://linkedlifedata.com/resource/pubmed/commentcorrection/19364659-15958542, http://linkedlifedata.com/resource/pubmed/commentcorrection/19364659-16260731, http://linkedlifedata.com/resource/pubmed/commentcorrection/19364659-16410314, http://linkedlifedata.com/resource/pubmed/commentcorrection/19364659-16464743, http://linkedlifedata.com/resource/pubmed/commentcorrection/19364659-16492735, http://linkedlifedata.com/resource/pubmed/commentcorrection/19364659-17078892, http://linkedlifedata.com/resource/pubmed/commentcorrection/19364659-1729377, http://linkedlifedata.com/resource/pubmed/commentcorrection/19364659-17590175, http://linkedlifedata.com/resource/pubmed/commentcorrection/19364659-18252253, http://linkedlifedata.com/resource/pubmed/commentcorrection/19364659-18287021, http://linkedlifedata.com/resource/pubmed/commentcorrection/19364659-18414668, http://linkedlifedata.com/resource/pubmed/commentcorrection/19364659-18566422, http://linkedlifedata.com/resource/pubmed/commentcorrection/19364659-9671764
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1096-0023
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
46
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
351-8
pubmed:dateRevised
2011-9-26
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Molecular characterization of IL-32 in human endothelial cells.
pubmed:affiliation
Department of Radiation Oncology, Vanderbilt-Ingram Cancer Center, Vanderbilt University Medical Center, 712 PRB, 2220 Pierce Ave., Nashville, TN 37232, USA.
pubmed:publicationType
Journal Article, Research Support, N.I.H., Extramural