Linked Life Data

19361962

Source:http://linkedlifedata.com/resource/pubmed/id/19361962

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2009-5-12
pubmed:abstractText
The direct electron transfer and electrocatalysis of hemoglobin (Hb) entrapped in polyvinyl alcohol (PVA)-room temperature ionic liquid (i.e., 1-octyl-3-methylimidazolium hexafluorophosphate [OMIM]PF(6)) composition has been investigated by using cyclic voltammetry and chronocoulometry. It is found that the composition can promote the direct electron transfer of Hb and the heterogeneous electron transfer rate constant (k(s)) of immobilized Hb is enhanced to 19.9 s(-1). The immobilized Hb also shows high electro-catalytic activity towards the redox of oxygen, hydrogen peroxide and nitrite. The Michaelis constants (K(m)) decrease to 1.2x10(-4) M (for hydrogen peroxide) and 9.4x10(-3) M (for nitrite). The surface concentration of electroactive Hb is estimated and it is ca. 1.4x10(-10) mol cm(-2), meaning that several layers of immobilized Hb take part in the electrochemical reaction. When gold nanoparticles (GNP) is introduced into the composition, the resulting PVA-GNP-[OMIM]PF(6) composition presents better performance. The electrochemical characteristic of immobilized Hb is improved further. Thus PVA-GNP-[OMIM]PF(6) composition is more suitable for the immobilization of Hb. Therefore, it is a good strategy to prepare novel composition for protein immobilization by using several materials with different function.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1873-4367
pubmed:author
pubmed:issnType
Electronic
pubmed:day
1
pubmed:volume
71
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
288-92
pubmed:dateRevised
2009-10-16
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Polyvinyl alcohol-ionic liquid composition for promoting the direct electron transfer and electrocatalysis of hemoglobin.
pubmed:affiliation
Department of Chemistry, Wuhan University, Wuhan 430072, PR China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't