Linked Life Data

19360785

Source:http://linkedlifedata.com/resource/pubmed/id/19360785

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2009-4-21
pubmed:abstractText
Microchip free flow IEF (muFFIEF) with monolithic IPG was proposed for protein prefractionation. The monolithic materials were first prepared by UV irradiation in a microchamber of 43 mm length, 23 mm width, and 38 mum depth. Carrier ampholytes (CAs) were further immobilized on the monolith by chemical bonding, to form a stable pH gradient. By such a technique, the continuous introduction of CAs in traditional muFFIEF could be avoided, not only to decrease the operation cost, but also to avoid the interference of CAs for the further protein identification by MS/MS. With a fluorescence microscope as the detector, under the optimal conditions, the separation of FITC labeled beta-lactoglobulin and carbonic anhydrase, with 0.9 unit difference on pI, was achieved with good reproducibility. The experimental results demonstrated that under the experimental conditions we applied, the separation mechanism of muFFIEF with M-IPG materials might be the cooperative effects of IEF and CZE, and the former one plays a predominant role.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1615-9314
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
32
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1211-5
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Microchip free flow isoelectric focusing for protein prefractionation using monolith with immobilized pH gradient.
pubmed:affiliation
School of Life Science & Technology, Beijing Institute of Technology, Beijing, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't