19359381

Source:http://linkedlifedata.com/resource/pubmed/id/19359381

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Predicate	Object
rdf:type	pubmed:Citation
lifeskim:mentions	umls-concept:C0006104, umls-concept:C0017262, umls-concept:C0017337, umls-concept:C0020792, umls-concept:C0025914, umls-concept:C0026809, umls-concept:C0040135, umls-concept:C0185117, umls-concept:C0443281, umls-concept:C0598509, umls-concept:C1537487, umls-concept:C2911684
pubmed:issue	8
pubmed:dateCreated	2009-7-22
pubmed:abstractText	Brain development is critically dependent on thyroid hormone (T(3)). Krüppel-like factor 9 (Klf9) is a T(3)-inducible gene in developing rat brain, and several lines of evidence support that KLF9 plays a key role in neuronal morphogenesis. Here we extend our findings to the mouse and demonstrate the presence of a functional T(3) response element (T(3)RE) in the 5' flanking region of the mouse Klf9 gene. Klf9 mRNA is strongly induced in the mouse hippocampus and cerebellum in a developmental stage- and T(3)-dependent manner. Computer analysis identified a near optimal direct repeat 4 (DR-4) T(3)RE 3.8 kb upstream of the Klf9 transcription start site, and EMSAs showed that T(3) receptor (TR)-retinoid X receptor heterodimers bound to the T(3)RE with high affinity. The T(3)RE acts as a strong positive response element in transfection assays using a minimal heterologous promoter. In the mouse neuroblastoma cell line N2a[TRbeta1], T(3) caused a dose-dependent up-regulation of Klf9 mRNA. Chromatin immunoprecipitation assays conducted with N2a[TRbeta1] cells showed that TRs associated with the Klf9 T(3)RE, and this association was promoted by T(3). Treatment of N2a[TRbeta1] cells with T(3) led to hyperacetylation of histones 3 and 4 at the T(3)RE site. Furthermore, TRs associated with the DR-4 T(3)RE in postnatal d 4 mouse brain, and histone 4 acetylation was greater at this site compared with other regions of the Klf9 gene. Our study identifies a functional DR-4 T(3)RE located in the mouse Klf9 gene to explain its regulation by T(3) during mammalian brain development.
pubmed:grant	http://linkedlifedata.com/resource/pubmed/grant/1R01NS046690-01, http://linkedlifedata.com/resource/pubmed/grant/R01 NS046690-01A2
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pubmed:language	eng
pubmed:journal	http://linkedlifedata.com/resource/pubmed/journal/0375040
pubmed:citationSubset	AIM
pubmed:chemical	http://linkedlifedata.com/resource/pubmed/chemical/Bteb1 protein, mouse, http://linkedlifedata.com/resource/pubmed/chemical/Histones, http://linkedlifedata.com/resource/pubmed/chemical/Kruppel-Like Transcription Factors, http://linkedlifedata.com/resource/pubmed/chemical/Thyroid Hormones, http://linkedlifedata.com/resource/pubmed/chemical/Triiodothyronine
pubmed:status	MEDLINE
pubmed:month	Aug
pubmed:issn	1945-7170
pubmed:author	pubmed-author:DenverRobert JRJ, pubmed-author:WilliamsonKeith EKE
pubmed:issnType	Electronic
pubmed:volume	150
pubmed:owner	NLM
pubmed:authorsComplete	Y
pubmed:pagination	3935-43
pubmed:dateRevised	2010-9-27
pubmed:meshHeading	pubmed-meshheading:19359381-Acetylation, pubmed-meshheading:19359381-Animals, pubmed-meshheading:19359381-Animals, Newborn, pubmed-meshheading:19359381-Brain, pubmed-meshheading:19359381-Cell Line, Tumor, pubmed-meshheading:19359381-Cerebellum, pubmed-meshheading:19359381-Chromatin Immunoprecipitation, pubmed-meshheading:19359381-Electrophoretic Mobility Shift Assay, pubmed-meshheading:19359381-Gene Expression Regulation, pubmed-meshheading:19359381-Hippocampus, pubmed-meshheading:19359381-Histones, pubmed-meshheading:19359381-Kruppel-Like Transcription Factors, pubmed-meshheading:19359381-Mice, pubmed-meshheading:19359381-Mice, Inbred C57BL, pubmed-meshheading:19359381-Response Elements, pubmed-meshheading:19359381-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:19359381-Thyroid Hormones, pubmed-meshheading:19359381-Transcription Initiation Site, pubmed-meshheading:19359381-Transfection, pubmed-meshheading:19359381-Triiodothyronine
pubmed:year	2009
pubmed:articleTitle	Identification of a thyroid hormone response element in the mouse Kruppel-like factor 9 gene to explain its postnatal expression in the brain.
pubmed:affiliation	Department of Molecular, Cellular, and Developmental Biology, The University of Michigan, Ann Arbor, Michigan 48109-1048, USA. rdenver@umich.edu
pubmed:publicationType	Journal Article, In Vitro

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