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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
2009-5-20
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pubmed:abstractText |
Therapeutic approaches aimed at targeting tumor surface markers using monoclonal antibodies provide a powerful strategy in cancer treatment. Here we report selection of single variable domains (VHH) of llama heavy chain antibodies, using a VHH-phage-display library. A reverse proteomic approach was used to identify the cognate proteins recognized by enriched VHH on HeLa cells. One of these VHH bound the integrin alpha 3 beta 1 (VLA-3) and was further characterized. Most interestingly, this VHH could inhibit VLA-3 mediated cell-matrix adhesion. Our approach provides a fast and efficient method to screen for novel cell surface markers on normal and tumor cells that may find diagnostic or therapeutic application in disease management or treatment.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
1872-9142
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
46
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2022-8
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pubmed:dateRevised |
2009-6-16
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pubmed:meshHeading |
pubmed-meshheading:19359042-Adhesiveness,
pubmed-meshheading:19359042-Amino Acid Sequence,
pubmed-meshheading:19359042-Animals,
pubmed-meshheading:19359042-Antibodies,
pubmed-meshheading:19359042-Camelids, New World,
pubmed-meshheading:19359042-Cell Adhesion,
pubmed-meshheading:19359042-Cell Membrane,
pubmed-meshheading:19359042-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:19359042-Extracellular Matrix,
pubmed-meshheading:19359042-Fluorescent Antibody Technique,
pubmed-meshheading:19359042-HeLa Cells,
pubmed-meshheading:19359042-Humans,
pubmed-meshheading:19359042-Immunoglobulin Heavy Chains,
pubmed-meshheading:19359042-Immunoglobulin Variable Region,
pubmed-meshheading:19359042-Immunoprecipitation,
pubmed-meshheading:19359042-Integrin alpha3beta1,
pubmed-meshheading:19359042-Keratinocytes,
pubmed-meshheading:19359042-Microscopy, Fluorescence,
pubmed-meshheading:19359042-Molecular Sequence Data,
pubmed-meshheading:19359042-Proteomics,
pubmed-meshheading:19359042-Reproducibility of Results
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pubmed:year |
2009
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pubmed:articleTitle |
Reverse proteomic antibody screening identifies anti adhesive VHH targeting VLA-3.
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pubmed:affiliation |
Department of Pathology, University Medical Center Utrecht, PO Box 85500, 3508 GA Utrecht, The Netherlands.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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