Source:http://linkedlifedata.com/resource/pubmed/id/19353243
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1-2
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| pubmed:dateCreated |
2009-12-3
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| pubmed:abstractText |
This study was designed to investigate whether indomethacin and NGX6 synergistically inhibit the growth and invasiveness of human colon cancer cells (HT-29 and SW620) and to elucidate the molecular mechanism of their action. Cell proliferation was assessed by MTT assay. Cell apoptosis was assessed by acridine orange/ethidium bromide staining (AO-EB) and annexin-V-FITC/PI assay. Invasive behaviors of colorectal cancer cells were examined by cell adhesion, migration, and invasion assays. Gap junctional intercellular communication (GJIC) was assessed by the scrape-loading/dye transfer technique. The subcellular localization and expression of beta-catenin protein was examined by immunofluorescence staining and western blot analysis, respectively. Indomethacin and NGX6 had a synergistic effect on inhibiting proliferation and invasiveness of colon cancer HT-29 and SW620 cells, restoring GJIC of HT-29 and SW620, and suppressing translocation of beta-catenin from the nucleus and cytoplasm to the plasma membrane. However, they did not have synergistic effects on enhancing apoptosis and suppressing extracellular matrix adhesion of HT-29 and SW620 cells. Indomethacin and NGX6 inhibit the proliferation and invasiveness of HT-29 and SW620 colon cancer cells by attenuating the WNT/ss-catenin signaling pathway.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Anti-Inflammatory Agents...,
http://linkedlifedata.com/resource/pubmed/chemical/Indomethacin,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/TMEM8B protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Suppressor Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Wnt Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/beta Catenin
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| pubmed:status |
MEDLINE
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| pubmed:month |
Oct
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| pubmed:issn |
1573-4919
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:volume |
330
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
71-81
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| pubmed:dateRevised |
2011-10-13
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| pubmed:meshHeading |
pubmed-meshheading:19353243-Anti-Inflammatory Agents, Non-Steroidal,
pubmed-meshheading:19353243-Apoptosis,
pubmed-meshheading:19353243-Cell Adhesion,
pubmed-meshheading:19353243-Cell Communication,
pubmed-meshheading:19353243-Cell Line, Tumor,
pubmed-meshheading:19353243-Cell Proliferation,
pubmed-meshheading:19353243-Colorectal Neoplasms,
pubmed-meshheading:19353243-Drug Synergism,
pubmed-meshheading:19353243-Gap Junctions,
pubmed-meshheading:19353243-Humans,
pubmed-meshheading:19353243-Indomethacin,
pubmed-meshheading:19353243-Membrane Proteins,
pubmed-meshheading:19353243-Neoplasm Invasiveness,
pubmed-meshheading:19353243-Signal Transduction,
pubmed-meshheading:19353243-Transfection,
pubmed-meshheading:19353243-Tumor Suppressor Proteins,
pubmed-meshheading:19353243-Wnt Proteins,
pubmed-meshheading:19353243-beta Catenin
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| pubmed:year |
2009
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| pubmed:articleTitle |
Synergistic effect of indomethacin and NGX6 on proliferation and invasion by human colorectal cancer cells through modulation of the Wnt/beta-catenin signaling pathway.
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| pubmed:affiliation |
Department of Digestion Medicine, The Third Affiliated Hospital of Xiang Ya School of Medicine, Central South University, Changsha, 410078, Hunan, People's Republic of China.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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