Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2009-4-24
pubmed:abstractText
We report here some efficient biotransformations using Escherichia coli strains with disruptions for the AcrAB-TolC efflux pump system. Biotransformations of compactin into pravastatin (6alpha-hydroxy-iso-compactin) were performed using E. coli strains with tolC and/or acrAB mutations expressing a cytochrome P450 (P450) gene. The production levels of pravastatin using strains with acrAB, tolC, and tolC acrAB mutations increased by 3.7-, 7.0-, and 7.1-fold, respectively. Likewise, the production levels of 25-hydroxy vitamin D3 and 25-hydroxy 4-cholesten 3-one using tolC acrAB mutant strains expressing an individual P450 gene increased by 2.2- and 16-fold, respectively. The enhancement of this biotransformation efficiency could be explained by increases in the intracellular amounts of substrates and the concentrations of active P450s. These results demonstrate that we have achieved versatile methods for efficient biotransformations using E. coli strains with tolC acrAB mutations expressing P450 genes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Outer Membrane Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Calcifediol, http://linkedlifedata.com/resource/pubmed/chemical/Cholecalciferol, http://linkedlifedata.com/resource/pubmed/chemical/Cholestenones, http://linkedlifedata.com/resource/pubmed/chemical/Cytochrome P-450 Enzyme System, http://linkedlifedata.com/resource/pubmed/chemical/Escherichia coli Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Lovastatin, http://linkedlifedata.com/resource/pubmed/chemical/Membrane Transport Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Pravastatin, http://linkedlifedata.com/resource/pubmed/chemical/cholest-4-en-3-one, http://linkedlifedata.com/resource/pubmed/chemical/compactin, http://linkedlifedata.com/resource/pubmed/chemical/tolC protein, E coli
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1347-6947
pubmed:author
pubmed:issnType
Electronic
pubmed:day
23
pubmed:volume
73
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
805-10
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:19352031-Bacterial Outer Membrane Proteins, pubmed-meshheading:19352031-Calcifediol, pubmed-meshheading:19352031-Cholecalciferol, pubmed-meshheading:19352031-Cholestenones, pubmed-meshheading:19352031-Cytochrome P-450 Enzyme System, pubmed-meshheading:19352031-Escherichia coli, pubmed-meshheading:19352031-Escherichia coli Proteins, pubmed-meshheading:19352031-Hydrophobic and Hydrophilic Interactions, pubmed-meshheading:19352031-Intracellular Space, pubmed-meshheading:19352031-Lovastatin, pubmed-meshheading:19352031-Membrane Transport Proteins, pubmed-meshheading:19352031-Mutation, pubmed-meshheading:19352031-Oxidation-Reduction, pubmed-meshheading:19352031-Pravastatin, pubmed-meshheading:19352031-Spectrum Analysis, pubmed-meshheading:19352031-Stereoisomerism, pubmed-meshheading:19352031-Substrate Specificity
pubmed:year
2009
pubmed:articleTitle
Efficient biotransformations using Escherichia coli with tolC acrAB mutations expressing cytochrome P450 genes.
pubmed:affiliation
Bioresource Laboratories, Mercian Corporation, 1808 Nakaizumi, Iwata, Shizuoka 438-0078, Japan. fujii-td@mercian.co.jp
pubmed:publicationType
Journal Article