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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2009-4-8
pubmed:abstractText
Fusarium asiaticum and F. graminearum are the primary causal agents of Fusarium head blight (FHB) of wheat in China. In this study, sensitivities of 159 F. asiaticum and F. graminearum isolates to a benzimidazole fungicide carbendazim (MBC) and to sterol demethylation inhibitors (DMIs) tebuconazole and prochloraz were determined. Among the 159 isolates, 9 were resistant to MBC and designated as MBC-R isolates. Three showed resistance to tebuconazole and prochloraz and designated as DMI-R isolates. There was no cross-resistance between MBC and DMI. Genetic analysis by microsatellite-primed polymerase chain reaction (PCR) showed that MBC-R or DMI-R isolates had different genotypes, which indicated that they originated from different wild-type parents. Analysis of two 14alpha-demethylase (cyp51) homologous genes (cyp51A and cyp51B) showed that the F. asiaticum isolates could be distinguished from F. graminearum isolates based on the sequence of cyp51A. Analysis of deduced amino acid sequence of cyp51A and cyp51B suggested that no mutations were associated with DMI resistance. Real-time PCR analysis showed that the DMI resistance was not related to the expression of cyp51A and cyp51B in F. asiaticum and F. graminearum, but expressions of both genes were induced greatly by the tebuconazole. Results of this study indicated that cyp51A would be an informative marker for analysis of population structure of F. asiaticum and F. graminearum, and the existence of homologous cyp51 genes in F. asiaticum and F. graminearum could provide new insights into DMI resistance in phytopathogenic fungi.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0031-949X
pubmed:author
pubmed:issnType
Print
pubmed:volume
99
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
487-97
pubmed:meshHeading
pubmed-meshheading:19351244-Amino Acid Sequence, pubmed-meshheading:19351244-Base Sequence, pubmed-meshheading:19351244-Benzimidazoles, pubmed-meshheading:19351244-Carbamates, pubmed-meshheading:19351244-China, pubmed-meshheading:19351244-Cytochrome P-450 Enzyme System, pubmed-meshheading:19351244-DNA, Fungal, pubmed-meshheading:19351244-DNA Fingerprinting, pubmed-meshheading:19351244-Drug Resistance, Fungal, pubmed-meshheading:19351244-Fungicides, Industrial, pubmed-meshheading:19351244-Fusarium, pubmed-meshheading:19351244-Gene Expression Regulation, Fungal, pubmed-meshheading:19351244-Genes, Fungal, pubmed-meshheading:19351244-Imidazoles, pubmed-meshheading:19351244-Microbial Sensitivity Tests, pubmed-meshheading:19351244-Molecular Sequence Data, pubmed-meshheading:19351244-Mycelium, pubmed-meshheading:19351244-Phylogeny, pubmed-meshheading:19351244-Triazoles, pubmed-meshheading:19351244-Triticum
pubmed:year
2009
pubmed:articleTitle
Characterization of sterol demethylation inhibitor-resistant isolates of Fusarium asiaticum and F. graminearum collected from wheat in China.
pubmed:affiliation
Institute of Biotechnology, Zhejiang University, Hangzhou, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't