Linked Life Data

19345543

Source:http://linkedlifedata.com/resource/pubmed/id/19345543

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2009-5-5
pubmed:abstractText
Simple, convenient, sensitive and accurate analytical methods are needed for the analysis of alkaloid components in Rhizoma Coptidis in traditional Chinese herbal medicine, which has important bioactivity. In the present study, a highly specific and sensitive method using (1)H NMR has been developed for the quantitative determination of protoberberine alkaloids berberine, palmatine, coptisine and jatrorrhizine in Coptis species and their commercial traditional Chinese medicine prescriptions. A (1)H NMR analysis of the H-13 signals of target protoberberine alkaloids was performed. By comparing the solvent effects on the resolution of these signals, methanol-d(4)-benzene-d(6) (75:25) is selected as an optimal (1)H NMR solvent. The quantity of the compounds is calculated by the relative ratio of the integral values of the target peak for each compound to the known amount of the internal standard anthracene. This method allows rapid and simple quantitation of protoberberine alkaloids from Coptis species and the more complex commercial prescriptions in 5 min without any pre-purification steps. The recoveries of these alkaloids from Coptis chinensis are in the range of 93-105%. Limit of detection of berberine in the plant material or prescription is 0.03 mg/mL. The advantages of this method are that no reference compounds are required for calibration curves, the quantification can be directly realized on a crude extract, and the better selectivity for protoberberine alkaloids and a very significant time-gain can be achieved, in comparison to conventional HPLC methods, for instance.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1873-264X
pubmed:author
pubmed:issnType
Electronic
pubmed:day
12
pubmed:volume
49
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1272-6
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
A rapid and simple determination of protoberberine alkaloids in Rhizoma Coptidis by 1H NMR and its application for quality control of commercial prescriptions.
pubmed:affiliation
Department of Applied Chemistry and Life Science, National PingTung University of Education, Pingtung 900, Taiwan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't