Linked Life Data

19344094

Source:http://linkedlifedata.com/resource/pubmed/id/19344094

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2009-4-6
pubmed:abstractText
The gemini quaternary salt (gemini-QUAT) containing two pyridinium residues per molecule, 3,3'-(2,7-dioxaoctane) bis (1-decylpyridinium bromide) (3DOBP-4,10), exerted fungicidal activity against Saccharomyces cerevisiae accompanied by respiration inhibition and the cytoplasmic material leakage of ATP, magnesium, and potassium ions. We previously found that gemini-QUAT exerted bacterioclastic action against Escherichia coli by causing the rapid and abundant leakage of turbid materials from the cells. In addition, the first stage of the bacterioclastic action was the leakage of magnesium ions, outer membrane protein E, ATP, and lipopolysaccharides. Here, we investigated how the gemini-QUAT 3DOBP-4,10 exerts fungicidal action against S. cerevislae. The results showed that that > or = 0.4 microM 3DOBP-4,10 stopped respiration and that > or = 3.0, 1.0 and 1.0 microM caused the leakage of cytoplasmic components ATP, magnesium and potassium ions, respectively. Scanning and transmission electron micrographs showed a preserved cell wall structure, whereas intracellular organelles were destroyed in cells incubated with 3DOBP-4,10. We postulated that 3DOBP-4,10 exerts its fungicidal action against S. cerevisiae not through cell wall destruction and protein leakage, but rather by penetrating the cell wall and disrupting the membranes of organelles.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1342-4815
pubmed:author
pubmed:issnType
Print
pubmed:volume
14
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
13-20
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
The mode of the antifungal activity of gemini-pyridinium salt against yeast.
pubmed:affiliation
Department of Biological Science and Technology, Biosystems Engineering, Institute of Technology and Science, The University of Tokushima Graduate School, 2-1, Minamijosanjima-cho, Tokushima 770-8506, Japan.
pubmed:publicationType
Journal Article