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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
Pt 4
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pubmed:dateCreated |
2009-4-3
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pubmed:abstractText |
DNA ligases catalyze the sealing of 5'-phosphate and 3'-hydroxyl termini at single-strand breaks in double-stranded DNA and their function is essential to maintain the integrity of the genome in DNA metabolism. An ATP-dependent DNA ligase from the archaeon Thermococcus sp. 1519 was overexpressed, purified and crystallized. Crystals were obtained using the hanging-drop vapour-diffusion method employing 35%(v/v) Tacsimate pH 7.0 as a precipitant and diffracted X-rays to 3.09 A resolution. They belonged to space group P4(1)2(1)2, with unit-cell parameters a = b = 79.7, c = 182.6 A.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/19342782-10871342,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19342782-11053387,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19342782-12486455,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19342782-15251207,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19342782-15299374,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19342782-15565146,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19342782-15572771,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19342782-16199559,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19342782-16820169,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19342782-17052461,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19342782-18262407,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19342782-18822770,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19342782-4377758
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
1744-3091
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
1
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pubmed:volume |
65
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
368-71
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pubmed:dateRevised |
2011-7-27
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pubmed:meshHeading |
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pubmed:year |
2009
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pubmed:articleTitle |
Overexpression, purification and crystallization of a thermostable DNA ligase from the archaeon Thermococcus sp. 1519.
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pubmed:affiliation |
Bach Institute of Biochemistry RAS, Leninsky Prospect 33, 119071 Moscow, Russia. eubez@yandex.ru
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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