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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1991-12-13
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pubmed:abstractText |
We have used an iterative in vitro genetic selection to identify the important structural features of the viral RNA element bound by the Rev protein of human immunodeficiency virus type 1 (HIV-1). Functional Rev-binding RNAs were selected from a pool of 10(13) variants of the wild-type Rev-binding domain. Bases conserved among the binding species define a 20 nucleotide core binding element. Covariation of some of these conserved bases indicates that the Rev-binding element is a stem-bulge-stem with a G:G base pair in the bulge. Mutational studies show that this non-Watson-Crick base pair is required for Rev binding in vitro and Rev responsiveness in vivo. We propose that the G:G base pair distorts the sugar-phosphate backbone of viral RNA and that this distortion is a critical determinant of recognition by Rev.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0092-8674
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
67
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pubmed:geneSymbol |
rev
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
529-36
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:1934059-Base Sequence,
pubmed-meshheading:1934059-Binding Sites,
pubmed-meshheading:1934059-DNA Mutational Analysis,
pubmed-meshheading:1934059-Gene Products, rev,
pubmed-meshheading:1934059-HIV-1,
pubmed-meshheading:1934059-Hydrogen Bonding,
pubmed-meshheading:1934059-Molecular Sequence Data,
pubmed-meshheading:1934059-Nucleic Acid Conformation,
pubmed-meshheading:1934059-Oligonucleotides,
pubmed-meshheading:1934059-Protein Binding,
pubmed-meshheading:1934059-RNA, Viral,
pubmed-meshheading:1934059-RNA-Binding Proteins,
pubmed-meshheading:1934059-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:1934059-Structure-Activity Relationship,
pubmed-meshheading:1934059-rev Gene Products, Human Immunodeficiency Virus
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pubmed:year |
1991
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pubmed:articleTitle |
HIV-1 Rev regulation involves recognition of non-Watson-Crick base pairs in viral RNA.
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pubmed:affiliation |
Department of Molecular Biology, Massachusetts General Hospital, Boston 02114.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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