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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2009-5-4
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pubmed:abstractText |
Positive selection of T-cell precursors is the process by which a diverse T-cell repertoire is established. Positive selection begins at the CD4(+)CD8(+) double positive (DP) stage of development and involves at least two steps. First, DP thymocytes down-regulate CD8 to become transitional single positive (TSP) CD4(+) thymocytes. Then, cells are selected to become either mature single positive CD4(+) or mature single positive CD8(+) thymocytes. We sought to define the function of Gads during the two steps of positive selection by analyzing a Gads-deficient mouse line. In Gads(+/+) mice, most TSP CD4(+) thymocytes are TCR(hi)Bcl-2(hi)CD69(+), suggesting that essential steps in positive selection occurred in the DP stage. Despite that Gads(-/-) mice could readily generate TSP CD4(+) thymocytes, many Gads(-/-) TSP CD4(+) cells were TCR(lo)Bcl-2(lo)CD69(-), suggesting that Gads(-/-) cells proceeded to the TSP CD4(+) stage prior to being positively selected. These data suggest that positive selection is not a prerequisite for the differentiation of DP thymocytes into TSP CD4(+) thymocytes. We propose a model in which positive selection and differentiation into the TSP CD4(+) stage are separable events and Gads is only required for positive selection.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/19337995-10209041,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/19337995-9872323
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
1521-4141
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
39
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1395-404
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pubmed:dateRevised |
2011-9-26
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pubmed:meshHeading |
pubmed-meshheading:19337995-Adaptor Proteins, Signal Transducing,
pubmed-meshheading:19337995-Animals,
pubmed-meshheading:19337995-Antigens, CD,
pubmed-meshheading:19337995-CD4-Positive T-Lymphocytes,
pubmed-meshheading:19337995-CD8-Positive T-Lymphocytes,
pubmed-meshheading:19337995-Cell Differentiation,
pubmed-meshheading:19337995-Flow Cytometry,
pubmed-meshheading:19337995-Immunophenotyping,
pubmed-meshheading:19337995-Mice,
pubmed-meshheading:19337995-Mice, Inbred C57BL,
pubmed-meshheading:19337995-Mice, Knockout,
pubmed-meshheading:19337995-Mice, Transgenic,
pubmed-meshheading:19337995-Receptors, Antigen, T-Cell, alpha-beta,
pubmed-meshheading:19337995-Specific Pathogen-Free Organisms
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pubmed:year |
2009
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pubmed:articleTitle |
Gads-deficient thymocytes are blocked at the transitional single positive CD4+ stage.
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pubmed:affiliation |
Department of Microbiology, Molecular Genetics, and Immunology, University of Kansas Medical Center, Kansas City, KS 66160, USA.
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pubmed:publicationType |
Journal Article
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