Source:http://linkedlifedata.com/resource/pubmed/id/19337668
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
2009-4-1
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pubmed:abstractText |
Photosystem I (PSI) is severely damaged by chilling at 4 degrees C in low light, especially in the chilling sensitive plant cucumber. To investigate the early events in PSI photoinhibition, we examined structural changes in the level of pigment-protein complexes in cucumber leaves in comparison with pea leaves. The complexes were separated on a native green gel and an increase in the intensity of a band was observed only in light-chilled cucumber leaves. The 77 K fluorescence emission spectrum of this green band indicated that the band was mainly composed of PSI with light-harvesting complex I. Each lane was cut from the green gel and separated on a fully denaturing SDS-PAGE in the second dimension. The new green gel band observed after light-chilling in cucumber leaves lacked 19, 18, and 16.5 kDa polypeptides. These results suggest that light-chilling facilitates the release of three peripheral polypeptides as an early event of chilling stress in vivo, which results in the inactivation of PSI in intact cucumber leaves.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
1474-905X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
8
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
535-41
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pubmed:meshHeading |
pubmed-meshheading:19337668-Cold Temperature,
pubmed-meshheading:19337668-Cucumis sativus,
pubmed-meshheading:19337668-Darkness,
pubmed-meshheading:19337668-Electrophoresis, Gel, Two-Dimensional,
pubmed-meshheading:19337668-Light,
pubmed-meshheading:19337668-Peas,
pubmed-meshheading:19337668-Peptides,
pubmed-meshheading:19337668-Photosynthesis,
pubmed-meshheading:19337668-Photosystem I Protein Complex,
pubmed-meshheading:19337668-Plant Leaves,
pubmed-meshheading:19337668-Plant Proteins,
pubmed-meshheading:19337668-Spectrometry, Fluorescence
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pubmed:year |
2009
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pubmed:articleTitle |
Loss of peripheral polypeptides in the stromal side of photosystem I by light-chilling in cucumber leaves.
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pubmed:affiliation |
Department of Molecular Biology, Pusan National University, Busan 609-735, Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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