Source:http://linkedlifedata.com/resource/pubmed/id/19334046
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
2009-5-11
|
| pubmed:databankReference | |
| pubmed:abstractText |
Cigarette smoking is the main risk factor for the development of squamous cell lung carcinoma (SCC). However, the smoking-related molecular changes in SCC have not been studied. Gene expression studies in both histologically normal bronchial epithelium and SCC epithelial samples identified genes differentially expressed between current and ex-smokers. Subsequently, expression levels of the smoking-related genes in normal bronchial epithelium were compared with those in SCC cells, since we hypothesized that the smoking-induced changes would be also deregulated in SCC. Gene expression profiles were generated using Agilent whole human genome microarrays on laser-microdissected normal bronchial epithelium and SCC samples. Expression levels of 246 genes, mainly related to oxidative stress response, were significantly different between normal bronchial epithelium of current and ex-smokers. Such a differential gene expression profile did not exist in SCC cells of smokers and ex-smokers. Interestingly, when comparing SCC and normal bronchial epithelium from ex-smokers, the vast majority of these 246 genes were also deregulated in SCC. When comparing SCC with normal epithelium from smokers, 22% of the up-regulated genes showed a similar high expression in SCC whereas 79% of the down-regulated genes were even further reduced in SCC as compared to current smokers. The down-regulated genes included several tumour suppressor genes, such as C9orf9, INHBB, LRIG1, SCGB3A1, SERPINI2, STEAP3 and ZMYND10. Thus, our study shows that the majority of genes up-regulated in normal bronchial epithelium of current smokers show similar high expression levels in SCC, while down-regulated genes are even further repressed in SCC. Our data indicate that smoking-related changes in normal bronchial epithelial cells persist in malignant transformed squamous cells.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:status |
MEDLINE
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| pubmed:month |
Jun
|
| pubmed:issn |
1096-9896
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| pubmed:author |
pubmed-author:BoelensMirjam CMC,
pubmed-author:FehrmannRudolf S NRS,
pubmed-author:GeerlingsMarieM,
pubmed-author:GroenHarry J MHJ,
pubmed-author:PostmaDirkje SDS,
pubmed-author:SietsmaHannieH,
pubmed-author:TimensWimW,
pubmed-author:de JongWouter KWK,
pubmed-author:te MeermanGerard JGJ,
pubmed-author:van den BergAnkeA
|
| pubmed:issnType |
Electronic
|
| pubmed:volume |
218
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
182-91
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| pubmed:meshHeading |
pubmed-meshheading:19334046-Aged,
pubmed-meshheading:19334046-Bronchi,
pubmed-meshheading:19334046-Carcinoma, Squamous Cell,
pubmed-meshheading:19334046-Case-Control Studies,
pubmed-meshheading:19334046-Female,
pubmed-meshheading:19334046-Gene Expression Profiling,
pubmed-meshheading:19334046-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:19334046-Humans,
pubmed-meshheading:19334046-Immunohistochemistry,
pubmed-meshheading:19334046-Lung Neoplasms,
pubmed-meshheading:19334046-Male,
pubmed-meshheading:19334046-Middle Aged,
pubmed-meshheading:19334046-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:19334046-Respiratory Mucosa,
pubmed-meshheading:19334046-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:19334046-Smoking,
pubmed-meshheading:19334046-Smoking Cessation
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| pubmed:year |
2009
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| pubmed:articleTitle |
Current smoking-specific gene expression signature in normal bronchial epithelium is enhanced in squamous cell lung cancer.
|
| pubmed:affiliation |
Department of Pathology, University Medical Centre Groningen, Groningen, The Netherlands.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|