Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
2009-5-13
pubmed:abstractText
Dppa4 (developmental pluripotency-associated 4) has been identified in several high-profile screens as a gene that is expressed exclusively in pluripotent cells. It encodes a nuclear protein with an SAP-like domain and appears to be associated preferentially with transcriptionally active chromatin. Its exquisite expression pattern and results of RNA interference experiments have led to speculation that Dppa4, as well as its nearby homolog Dppa2, might play essential roles in embryonic stem (ES) cell function and/or germ cell development. To rigorously assess suggested roles, we have generated Dppa4-deficient and Dppa4/Dppa2 doubly deficient ES cells, as well as mice lacking Dppa4. Contrary to predictions, we find that Dppa4 is completely dispensable for ES cell identity and germ cell development. Instead, loss of Dppa4 in mice results in late embryonic/perinatal death and striking skeletal defects with partial penetrance. Thus, surprisingly, Dppa4-deficiency affects tissues that apparently never transcribed the gene, and at least some loss-of-function defects manifest phenotypically at an embryonic stage long after physiologic Dppa4 expression has ceased. Concomitant with targeted gene inactivation, we have introduced into the Dppa4 locus a red fluorescent marker (tandem-dimer red fluorescent protein) that is compatible with green fluorescent proteins and allows noninvasive visualization of pluripotent cells and reprogramming events.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/19332562-10694879, http://linkedlifedata.com/resource/pubmed/commentcorrection/19332562-11835670, http://linkedlifedata.com/resource/pubmed/commentcorrection/19332562-12060735, http://linkedlifedata.com/resource/pubmed/commentcorrection/19332562-12228720, http://linkedlifedata.com/resource/pubmed/commentcorrection/19332562-12514105, http://linkedlifedata.com/resource/pubmed/commentcorrection/19332562-12620990, http://linkedlifedata.com/resource/pubmed/commentcorrection/19332562-12787504, http://linkedlifedata.com/resource/pubmed/commentcorrection/19332562-12787505, http://linkedlifedata.com/resource/pubmed/commentcorrection/19332562-12874139, http://linkedlifedata.com/resource/pubmed/commentcorrection/19332562-14595015, http://linkedlifedata.com/resource/pubmed/commentcorrection/19332562-14654002, http://linkedlifedata.com/resource/pubmed/commentcorrection/19332562-15018652, http://linkedlifedata.com/resource/pubmed/commentcorrection/19332562-15378075, http://linkedlifedata.com/resource/pubmed/commentcorrection/19332562-15673569, http://linkedlifedata.com/resource/pubmed/commentcorrection/19332562-16050252, http://linkedlifedata.com/resource/pubmed/commentcorrection/19332562-16153702, http://linkedlifedata.com/resource/pubmed/commentcorrection/19332562-16324220, http://linkedlifedata.com/resource/pubmed/commentcorrection/19332562-16621925, http://linkedlifedata.com/resource/pubmed/commentcorrection/19332562-16767105, http://linkedlifedata.com/resource/pubmed/commentcorrection/19332562-16904174, http://linkedlifedata.com/resource/pubmed/commentcorrection/19332562-16990585, http://linkedlifedata.com/resource/pubmed/commentcorrection/19332562-17068183, http://linkedlifedata.com/resource/pubmed/commentcorrection/19332562-17143267, http://linkedlifedata.com/resource/pubmed/commentcorrection/19332562-17171761, http://linkedlifedata.com/resource/pubmed/commentcorrection/19332562-17215298, http://linkedlifedata.com/resource/pubmed/commentcorrection/19332562-17291332, http://linkedlifedata.com/resource/pubmed/commentcorrection/19332562-17352659, http://linkedlifedata.com/resource/pubmed/commentcorrection/19332562-17475814, http://linkedlifedata.com/resource/pubmed/commentcorrection/19332562-17635532, http://linkedlifedata.com/resource/pubmed/commentcorrection/19332562-17855347, http://linkedlifedata.com/resource/pubmed/commentcorrection/19332562-18059259, http://linkedlifedata.com/resource/pubmed/commentcorrection/19332562-18097409, http://linkedlifedata.com/resource/pubmed/commentcorrection/19332562-18203677, http://linkedlifedata.com/resource/pubmed/commentcorrection/19332562-18295569, http://linkedlifedata.com/resource/pubmed/commentcorrection/19332562-18295582, http://linkedlifedata.com/resource/pubmed/commentcorrection/19332562-18366076, http://linkedlifedata.com/resource/pubmed/commentcorrection/19332562-18414030, http://linkedlifedata.com/resource/pubmed/commentcorrection/19332562-18433507, http://linkedlifedata.com/resource/pubmed/commentcorrection/19332562-18585351, http://linkedlifedata.com/resource/pubmed/commentcorrection/19332562-1948049, http://linkedlifedata.com/resource/pubmed/commentcorrection/19332562-2133553, http://linkedlifedata.com/resource/pubmed/commentcorrection/19332562-7501018, http://linkedlifedata.com/resource/pubmed/commentcorrection/19332562-8378314, http://linkedlifedata.com/resource/pubmed/commentcorrection/19332562-8398989, http://linkedlifedata.com/resource/pubmed/commentcorrection/19332562-8559668, http://linkedlifedata.com/resource/pubmed/commentcorrection/19332562-9661200, http://linkedlifedata.com/resource/pubmed/commentcorrection/19332562-9814708
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1098-5549
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
29
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3186-203
pubmed:dateRevised
2010-9-22
pubmed:meshHeading
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