Linked Life Data

19332019

Source:http://linkedlifedata.com/resource/pubmed/id/19332019

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2009-5-4
pubmed:abstractText
Western blot analysis has been a useful method for analysis of expression levels of specific proteins and is conducted after sodium dodecyl sulfate (SDS) or native polyacrylamide gel electrophoresis without staining the gel. However, when it is necessary to analyze the gel, duplicate polyacrylamide gels usually must be prepared, one of which is stained, leading to the consumption of precious sample. Thus, we developed a convenient and efficient Western blotting method using a stained gel. This simple modification should be beneficial for analyzing samples that are limited in quantity and/or samples for which the stained gel serves as the loading control.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1096-0309
pubmed:author
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
389
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
171-3
pubmed:dateRevised
2010-3-22
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Application of SYPRO Ruby- and Flamingo-stained polyacrylamide gels to Western blot analysis.
pubmed:affiliation
Department of Agricultural Chemistry, Graduate School of Agriculture, Tokyo University of Agriculture, Setagaya-ku, Tokyo 156-8502, Japan.
pubmed:publicationType
Journal Article