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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
2009-7-29
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pubmed:abstractText |
One of the 14-3-3 protein isoforms, 14-3-3epsilon, was previously shown to be increased during skin aging. We suggest here a possible role for the 14-3-3epsilon protein in skin aging by providing evidence that 14-3-3epsilon increases the expression of the matrix-metalloproteinase (MMP)-2 gene in NIH3T3 fibroblast cells. Expression of the 14-3-3epsilon gene in NIH3T3 cells primarily up-regulated the expression of the MMP-2 gene at the transcriptional level by inducing specific DNA binding proteins bound to an upstream region of the MMP-2 promoter from -1,629 to -1,612. Inhibition of endogenous 14-3-3epsilon gene expression by RNA interference also decreased endogenous MMP-2 gene expression. Furthermore, up-regulation of the MMP-2 gene by 14-3-3epsilon was suppressed by expression of a dominant-negative mutant of p38 MAP kinase. These findings strongly suggest that increased expression of 14-3-3epsilon contributes to remodeling of extracellular matrix in skin through increasing MMP-2 gene expression via p38 MAP kinase signaling.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/19322035-10419448,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19322035-10506168,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19322035-10692106,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19322035-10788521,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19322035-10836149,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19322035-11205746,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19322035-11598960,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19322035-11710936,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19322035-11994741,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19322035-12208239,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19322035-12535209,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19322035-12730128,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19322035-12796778,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19322035-15140222,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19322035-15350836,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/19322035-15886528,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19322035-15888315,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19322035-16098031,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19322035-1664713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19322035-17543885,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19322035-17728103,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19322035-17934334,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19322035-8552187,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/19322035-9358139
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
1226-3613
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
31
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pubmed:volume |
41
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
453-561
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:19322035-14-3-3 Proteins,
pubmed-meshheading:19322035-Animals,
pubmed-meshheading:19322035-Electrophoretic Mobility Shift Assay,
pubmed-meshheading:19322035-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:19322035-Matrix Metalloproteinase 2,
pubmed-meshheading:19322035-Mice,
pubmed-meshheading:19322035-NIH 3T3 Cells,
pubmed-meshheading:19322035-Plasmids,
pubmed-meshheading:19322035-Promoter Regions, Genetic,
pubmed-meshheading:19322035-RNA, Messenger,
pubmed-meshheading:19322035-RNA, Small Interfering,
pubmed-meshheading:19322035-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:19322035-Signal Transduction,
pubmed-meshheading:19322035-Transfection,
pubmed-meshheading:19322035-p38 Mitogen-Activated Protein Kinases
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pubmed:year |
2009
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pubmed:articleTitle |
14-3-3epsilon protein increases matrix metalloproteinase-2 gene expression via p38 MAPK signaling in NIH3T3 fibroblast cells.
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pubmed:affiliation |
Department of Biological Science, Sungkyunkwan University, Suwon 440-746, Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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