Source:http://linkedlifedata.com/resource/pubmed/id/19318257
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
8
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pubmed:dateCreated |
2009-4-13
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pubmed:abstractText |
Sesquiterpene lactones (SQTLs) have been shown to suppress the degranulation as inferred by histamine release in rat basophilic leukemia RBL-2H3 cells. In this study, we isolated the 9 kinds of SQTLs from Eupatorium chinense L. and examined the effects of these SQTLs on the degranulation in RBL-2H3 cells. The chemical structures of two novel compounds (SQTL-3 and 8) were determined. All the SQTLs suppressed the degranulation from Ag-stimulated RBL-2H3 cells. To disclose the inhibitory mechanism of degranulation by SQTLs, we examined the activation of intracellular signaling molecules such as Lyn, Syk, and PLCgammas and intracellular free Ca(2+) concentration ([Ca(2+)]i). None of these SQTLs showed the activation of Syk and PLCgammas. The intracellular free Ca(2+) concentration ([Ca(2+)]i) was elevated by Fc epsilonRI activation, but SQTLs treatment reduced the elevation of [Ca(2+)]i by suppressing Ca(2+) influx. Thus, it was suggested that the suppression of Ag-stimulated degranulation by these SQTLs is mainly due to the decreased Ca(2+) influx. Furthermore, in order to clarify the in vivo effect of SQTL-rich extract, we administered SQTL-rich extract to the type I allergic model mice and measured the passive cutaneous anaphylaxis (PCA) reaction induced by IgE-antigen complex. The SQTLs remarkably suppressed PCA reaction in a dose-dependent manner. Thus, it was suggested that SQTLs would be a candidate as an anti-allergic agent.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin E,
http://linkedlifedata.com/resource/pubmed/chemical/Lactones,
http://linkedlifedata.com/resource/pubmed/chemical/Reactive Oxygen Species,
http://linkedlifedata.com/resource/pubmed/chemical/Sesquiterpenes
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
1464-3391
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pubmed:author | |
pubmed:issnType |
Electronic
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pubmed:day |
15
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pubmed:volume |
17
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3189-97
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pubmed:meshHeading |
pubmed-meshheading:19318257-Animals,
pubmed-meshheading:19318257-Basophil Degranulation Test,
pubmed-meshheading:19318257-Basophils,
pubmed-meshheading:19318257-Calcium,
pubmed-meshheading:19318257-Cell Degranulation,
pubmed-meshheading:19318257-Eupatorium,
pubmed-meshheading:19318257-Histamine Release,
pubmed-meshheading:19318257-Immunoglobulin E,
pubmed-meshheading:19318257-Lactones,
pubmed-meshheading:19318257-Leukemia, Basophilic, Acute,
pubmed-meshheading:19318257-Magnetic Resonance Spectroscopy,
pubmed-meshheading:19318257-Mice,
pubmed-meshheading:19318257-Passive Cutaneous Anaphylaxis,
pubmed-meshheading:19318257-Rats,
pubmed-meshheading:19318257-Reactive Oxygen Species,
pubmed-meshheading:19318257-Sesquiterpenes,
pubmed-meshheading:19318257-Signal Transduction
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pubmed:year |
2009
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pubmed:articleTitle |
Inhibitory effects of sesquiterpene lactones isolated from Eupatorium chinense L. on IgE-mediated degranulation in rat basophilic leukemia RBL-2H3 cells and passive cutaneous anaphylaxis reaction in mice.
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pubmed:affiliation |
Gifu International Institute of Biotechnology, 1-1 Naka-Fudogaoka, Kakamigahara, Gifu 504-0838, Japan. titoh@giib.or.jp
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pubmed:publicationType |
Journal Article
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