Linked Life Data

1930695

Source:http://linkedlifedata.com/resource/pubmed/id/1930695

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1991-12-19
pubmed:databankReference
pubmed:abstractText
A Caenorhabditis elegans lambda ZAP cDNA library was screened using a fragment amplified from highly conserved regions of the mammalian and Drosophila elongation factor 2 (EF-2). Two types of cDNA clones were obtained, corresponding to two mRNA species with 3'-untranslated regions of 60 and 115 nucleotides, both encoding identical polypeptides. Sequence analysis of these clones and comparisons with hamster and Drosophila EF-2 sequences suggests that they encode C. elegans EF-2. Clone pCef6A, encoding the entire C. elegans EF-2 mRNA sequence including 45 nucleotides of 5'-untranslated region, contains a 2,556-bp open reading frame which predicts a polypeptide of 852 amino acid residues (Mr 94,564). The deduced amino acid sequence is greater than 80% identical to that of mammalian and Drosophila EF-2. Conserved sequence segments shared among a variety of GTP-binding proteins are found in the amino-terminal region. The carboxy-terminal half contains segments unique to EF-2 and its prokaryotic homolog, EF-G, as well as the histidyl residue which is ADP-ribosylated by diphtheria toxin. The C. elegans protein contains a 12-amino-acid insertion between positions 90 and 100, and a 13-amino-acid deletion between positions 237 and 260, relative to hamster EF-2. Partial sequencing of a genomic clone encoding the entire C. elegans EF-2 gene (named eft-2) has so far revealed two introns of 48 and 44 bp following codons Gln-191 and Gln-250, respectively. Southern and Northern blot analyses indicate that eft-2 is a single-copy gene and encodes a 3-kb mRNA species which is present throughout nematode development.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1044-5498
pubmed:author
pubmed:issnType
Print
pubmed:volume
10
pubmed:geneSymbol
eft-1
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
603-11
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:1930695-Amino Acid Sequence, pubmed-meshheading:1930695-Animals, pubmed-meshheading:1930695-Base Sequence, pubmed-meshheading:1930695-Blotting, Northern, pubmed-meshheading:1930695-Blotting, Southern, pubmed-meshheading:1930695-Caenorhabditis, pubmed-meshheading:1930695-Cloning, Molecular, pubmed-meshheading:1930695-DNA, pubmed-meshheading:1930695-Molecular Sequence Data, pubmed-meshheading:1930695-Peptide Elongation Factor 2, pubmed-meshheading:1930695-Peptide Elongation Factors, pubmed-meshheading:1930695-Polymerase Chain Reaction, pubmed-meshheading:1930695-Protein Biosynthesis, pubmed-meshheading:1930695-RNA, Messenger, pubmed-meshheading:1930695-Restriction Mapping, pubmed-meshheading:1930695-Sequence Alignment, pubmed-meshheading:1930695-Sequence Homology, Nucleic Acid
pubmed:year
1991
pubmed:articleTitle
Molecular cloning and characterization of the Caenorhabditis elegans elongation factor 2 gene (eft-2).
pubmed:affiliation
Department of Biochemistry, Faculty of Medicine, University of British Columbia, Vancouver, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't