Linked Life Data

19305933

Source:http://linkedlifedata.com/resource/pubmed/id/19305933

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2009-3-23
pubmed:abstractText
Simple and sensitive chemiluminescence (CL) and CL imaging methods have been developed for the magnetic bead-based DNA hybridization assay. The assay relies on the high sensitivity and long stable light signal of the CL system in which horseradish peroxidase (HRP) catalyzes the luminol-H(2)O(2) reaction with para-iodophenol (PIP) as the enhancer. In this protocol, a sandwich DNA hybridization is performed by mixing the target DNA with the magnetic bead-captured DNA and the biotinylated reporter DNA, followed through the biotin-streptavidin reaction with conjugated HRP, and then the conjugated HRP is determined by the CL system. The proposed CL protocol is suitable for for the detection of sequence-specific DNA related to the avian influenza A H1N1 virus at levels as low as 10 amol, and the CL imaging detection has a similar sensitivity. The sensitivities of the proposed methods with the HRP label are better than most of the metal nanoparticle-based methods, and are comparable with that of utilizing amplified techniques for DNA hybridization detection. In addition, the perfectly complementary DNA sequences and the single-base mismatched DNA sequences can be better distinguished by a thermally-stringent hybridization and washing steps. So, the proposed CL method can offer great promise for single-nucleotide polymorphism (SNP) analysis. Moreover, the proposed method may have significant potential for the simultaneous detection of various DNA sequences when different capture DNA sequences and reporter DNA sequences are used in a microarray.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1364-5528
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
134
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
800-4
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Magnetic bead-based DNA hybridization assay with chemiluminescence and chemiluminescent imaging detection.
pubmed:affiliation
College of Chemistry & Molecular Sciences, Wuhan University, Wuhan, 430072, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't