Linked Life Data

19301412

Source:http://linkedlifedata.com/resource/pubmed/id/19301412

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2009-6-11
pubmed:abstractText
It was observed that a biocatalyst prepared from dehydrated whole cells of a recombinant Escherichia coli (initially suspended in borate buffer) was able to hydrolyze gaseous 1-chlorobutane in a solid/gas reactor. Nevertheless, at 40 degrees C and for a 0.7 water activity, it rapidly lost its activity. The explanation of this phenomenon was first investigated by observing the biocatalyst structure at the microscopic level and by studying the localization of the dehalogenase involved in catalysis (intracellular/extracellular). The behavior of this biocatalyst was then compared with that of a preparation made from cells extracts. The reasons of the inactivation are discussed in terms of thermal denaturation and protective effect of buffer salts.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1097-0290
pubmed:author
pubmed:copyrightInfo
(c) 2009 Wiley Periodicals, Inc.
pubmed:issnType
Electronic
pubmed:day
1
pubmed:volume
103
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
687-95
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Dehalogenation of gaseous 1-chlorobutane by dehydrated whole cells: Influence of the microenvironment of the halidohydrolase on the stability of the biocatalyst.
pubmed:affiliation
LIENSs-LIttoral Environnement et Sociétés, Université de La Rochelle-UMR 6250-CNRS, Avenue Michel Crépeau, 17042 La Rochelle Cedex 1, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't