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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1991-11-12
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pubmed:abstractText |
The surfactant proteins SP-A and SP-D were obtained from rats given intratracheal instillation of silica. SP-D was isolated from the 33,000 g supernatant of rat bronchoalveolar lavage fluids, and we examined whether SP-D affects surfactant secretion by alveolar type II cells. Native SP-D affected neither basal secretion nor stimulated secretion by type II cells. However, native SP-D counteracted the inhibitory effect of SP-A on surfactant secretion in a concentration-dependent manner; however, SP-D failed to counteract the inhibitory effect of concanavalin A. The activity of SP-D was unaffected by inclusion of excess methyl alpha-mannoside. Excess native SP-D competed with 125I-SP-A for high-affinity binding to type II cells. Heat treatment of SP-D and antibody against SP-D both decreased SP-D activity. Butanol extraction of native SP-D was most effective at destroying SP-D activity and attenuated the ability of the protein to compete with labelled SP-A for binding to type II cells. The butanol-soluble fraction of SP-D possessed the ability to alter the inhibitory effect of SP-A to the same extent as native SP-D. Direct binding of 125I-SP-A on nitrocellulose sheets demonstrated that SP-A could bind native SP-D, but not butanol-extracted SP-D. We conclude that native SP-D alters SP-A activity in type II cells through interaction with it via SP-D-associated lipids.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1930130-1175787,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1930130-13641241,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1930130-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1930130-1993679,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1930130-2108147,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1930130-2449439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1930130-2501785,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1930130-2675969,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1930130-2820982,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1930130-2840667,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1930130-2846572,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1930130-2995386,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1930130-3006655,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1930130-3021734,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1930130-3034252,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1930130-3219363,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1930130-3337835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1930130-34631,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1930130-3469643,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1930130-3654429,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1930130-3818626,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1930130-3922400,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1930130-4733239,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1930130-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1930130-6688420
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Methylmannosides,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylcholines,
http://linkedlifedata.com/resource/pubmed/chemical/Phospholipids,
http://linkedlifedata.com/resource/pubmed/chemical/Proteolipids,
http://linkedlifedata.com/resource/pubmed/chemical/Pulmonary Surfactant-Associated...,
http://linkedlifedata.com/resource/pubmed/chemical/Pulmonary Surfactant-Associated...,
http://linkedlifedata.com/resource/pubmed/chemical/Pulmonary Surfactant-Associated...,
http://linkedlifedata.com/resource/pubmed/chemical/Pulmonary Surfactants,
http://linkedlifedata.com/resource/pubmed/chemical/Tetradecanoylphorbol Acetate,
http://linkedlifedata.com/resource/pubmed/chemical/methylmannoside
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
279 ( Pt 1)
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
115-9
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:1930130-Animals,
pubmed-meshheading:1930130-Binding, Competitive,
pubmed-meshheading:1930130-Blotting, Western,
pubmed-meshheading:1930130-Bronchoalveolar Lavage Fluid,
pubmed-meshheading:1930130-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:1930130-Glycoproteins,
pubmed-meshheading:1930130-Methylmannosides,
pubmed-meshheading:1930130-Phosphatidylcholines,
pubmed-meshheading:1930130-Phospholipids,
pubmed-meshheading:1930130-Proteolipids,
pubmed-meshheading:1930130-Pulmonary Alveoli,
pubmed-meshheading:1930130-Pulmonary Surfactant-Associated Protein A,
pubmed-meshheading:1930130-Pulmonary Surfactant-Associated Protein D,
pubmed-meshheading:1930130-Pulmonary Surfactant-Associated Proteins,
pubmed-meshheading:1930130-Pulmonary Surfactants,
pubmed-meshheading:1930130-Rats,
pubmed-meshheading:1930130-Rats, Inbred Strains,
pubmed-meshheading:1930130-Tetradecanoylphorbol Acetate
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pubmed:year |
1991
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pubmed:articleTitle |
Surfactant protein D (SP-D) counteracts the inhibitory effect of surfactant protein A (SP-A) on phospholipid secretion by alveolar type II cells. Interaction of native SP-D with SP-A.
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pubmed:affiliation |
Department of Biochemistry, Sapporo Medical College, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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