Linked Life Data

19301030

Source:http://linkedlifedata.com/resource/pubmed/id/19301030

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2009-4-27
pubmed:abstractText
In yeast and mammals, ATP-dependent chromatin remodelling complexes of the SWI/SNF family play critical roles in the regulation of transcription, cell proliferation, differentiation and development. Homologues of conserved subunits of SWI/SNF-type complexes, including Snf2-type ATPases and SWI3-type proteins, participate in analogous processes in Arabidopsis. Recent studies indicate a remarkable similarity between phenotypic effects of mutations in the SWI3 homologue ATSWI3C and bromodomain-ATPase BRM genes. To verify the extent of functional similarity between BRM and ATSWI3C, we have constructed atswi3c brm double mutants and compared their phenotypic traits to those of simultaneously grown single atswi3c and brm mutants. In addition to inheritance of characteristic developmental abnormalities shared by atswi3c and brm mutants, some additive brm-specific traits were also observed in the atswi3c brm double mutants. Unlike atswi3c, the brm mutation results in the enhancement of abnormal carpel development and pollen abortion leading to complete male sterility. Despite the overall similarity of brm and atswi3c phenotypes, a critical requirement for BRM in the differentiation of reproductive organs suggests that its regulatory functions do not entirely overlap those of ATSWI3C. The detection of two different transcript isoforms indicates that BRM is regulated by alternative splicing that creates an in-frame premature translation stop codon in its SNF2-like ATPase coding domain. The analysis of Arabidopsis mutants in nonsense-mediated decay suggests an involvement of this pathway in the control of alternative BRM transcript level.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1432-2048
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
229
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1281-92
pubmed:meshHeading
pubmed-meshheading:19301030-Adenosine Triphosphatases, pubmed-meshheading:19301030-Alternative Splicing, pubmed-meshheading:19301030-Arabidopsis Proteins, pubmed-meshheading:19301030-Blotting, Northern, pubmed-meshheading:19301030-Blotting, Western, pubmed-meshheading:19301030-DNA, Bacterial, pubmed-meshheading:19301030-DNA-Binding Proteins, pubmed-meshheading:19301030-Flowers, pubmed-meshheading:19301030-Gene Expression Profiling, pubmed-meshheading:19301030-Gene Expression Regulation, Plant, pubmed-meshheading:19301030-Microscopy, Electron, Scanning, pubmed-meshheading:19301030-Mutagenesis, Insertional, pubmed-meshheading:19301030-Mutation, pubmed-meshheading:19301030-Phenotype, pubmed-meshheading:19301030-Pollen, pubmed-meshheading:19301030-Protein Subunits, pubmed-meshheading:19301030-RNA-Binding Proteins, pubmed-meshheading:19301030-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:19301030-Suppression, Genetic
pubmed:year
2009
pubmed:articleTitle
Genetic analysis of functional redundancy of BRM ATPase and ATSWI3C subunits of Arabidopsis SWI/SNF chromatin remodelling complexes.
pubmed:affiliation
Laboratory of Plant Molecular Biology, University of Warsaw, Pawinskiego 5A, 02-106, Warsaw, Poland.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't