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rdf:type |
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lifeskim:mentions |
umls-concept:C0010453,
umls-concept:C0017262,
umls-concept:C0030956,
umls-concept:C0031150,
umls-concept:C0039198,
umls-concept:C0063710,
umls-concept:C0086418,
umls-concept:C0185117,
umls-concept:C0332307,
umls-concept:C1413323,
umls-concept:C1416395,
umls-concept:C1416396,
umls-concept:C1416467,
umls-concept:C1879547,
umls-concept:C1998793,
umls-concept:C2911684
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pubmed:issue |
21
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pubmed:dateCreated |
2009-5-27
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pubmed:abstractText |
Although adoptive transfer of regulatory T cells (Foxp3(+) Tregs) has proven to be efficacious in the prevention and treatment of autoimmune diseases and graft-versus-host disease in rodents, a major obstacle for the use of Treg immunotherapy in humans is the difficulty of obtaining a highly purified preparation after ex vivo expansion. We have identified latency-associated peptide (LAP) and IL-1 receptor type I and II (CD121a/CD121b) as unique cell-surface markers that distinguish activated Tregs from activated FOXP3(-) and FOXP3(+) non-Tregs. We show that it is feasible to sort expanded FOXP3(+) Tregs from non-Tregs with the use of techniques for magnetic bead cell separation based on expression of these 3 markers. After separation, the final product contains greater than 90% fully functional FOXP3(+) Tregs. This novel protocol should facilitate the purification of Tregs for both cell-based therapies as well as detailed studies of human Treg function in health and disease.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/19299332-10795741,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19299332-11466340,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19299332-14707053,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19299332-15090447,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19299332-15374887,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19299332-15616015,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19299332-15790359,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19299332-15790361,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19299332-15870014,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19299332-16412790,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19299332-16818676,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19299332-16818678,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19299332-16903903,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19299332-16917003,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19299332-16920638,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19299332-16955142,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19299332-17067661,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19299332-17136045,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19299332-17182569,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19299332-17277168,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19299332-17371972,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19299332-17452902,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19299332-17453966,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19299332-17463169,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19299332-17511675,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19299332-17644734,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19299332-17653126,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19299332-17653127,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19299332-17713426,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19299332-17878367,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19299332-18077005,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19299332-18285775,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19299332-18424697,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19299332-18490420,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19299332-18710931,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19299332-18776930,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19299332-18941119,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19299332-19074986
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Biological Markers,
http://linkedlifedata.com/resource/pubmed/chemical/FOXP3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Forkhead Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/IL1R2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-1 Type I,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-1 Type II
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
1528-0020
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
21
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pubmed:volume |
113
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5125-33
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pubmed:dateRevised |
2010-9-23
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pubmed:meshHeading |
pubmed-meshheading:19299332-Biological Markers,
pubmed-meshheading:19299332-Cell Culture Techniques,
pubmed-meshheading:19299332-Cell Proliferation,
pubmed-meshheading:19299332-Forkhead Transcription Factors,
pubmed-meshheading:19299332-Humans,
pubmed-meshheading:19299332-Immunomagnetic Separation,
pubmed-meshheading:19299332-Receptors, Cell Surface,
pubmed-meshheading:19299332-Receptors, Interleukin-1 Type I,
pubmed-meshheading:19299332-Receptors, Interleukin-1 Type II,
pubmed-meshheading:19299332-T-Lymphocytes, Regulatory
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pubmed:year |
2009
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pubmed:articleTitle |
Selective expression of latency-associated peptide (LAP) and IL-1 receptor type I/II (CD121a/CD121b) on activated human FOXP3+ regulatory T cells allows for their purification from expansion cultures.
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pubmed:affiliation |
Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, USA. dtran@niaid.nih.gov
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Intramural
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