Source:http://linkedlifedata.com/resource/pubmed/id/19298834
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2009-5-18
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| pubmed:abstractText |
Antimicrobial peptides are components of innate immunity that is the first-line defense against invading pathogens for a wide range of organisms. Here, we describe the isolation, biological characterization and amino acid sequencing of a novel neutral Glycine/Leucine-rich antimicrobial peptide from skin secretion of Leptodactylus pentadactylus named leptoglycin. The amino acid sequence of the peptide purified by RP-HPLC (C(18) column) was deduced by mass spectrometric de novo sequencing and confirmed by Edman degradation: GLLGGLLGPLLGGGGGGGGGLL. Leptoglycin was able to inhibit the growth of Gram-negative bacteria Pseudomonas aeruginosa, Escherichia coli and Citrobacter freundii with minimal inhibitory concentrations (MICs) of 8 microM, 50 microM, and 75 microM respectively, but it did not show antimicrobial activity against Gram-positive bacteria (Staphylococcus aureus, Micrococcus luteus and Enterococcus faecalis), yeasts (Candida albicans and Candida tropicalis) and dermatophytes fungi (Microsporum canis and Trichophyton rubrum). No hemolytic activity was observed at the 2-200 microM range concentration. The amino acid sequence of leptoglycin with high level of glycine (59.1%) and leucine (36.4%) containing an unusual central proline suggests the existence of a new class of Gly/Leu-rich antimicrobial peptides. Taken together, these results suggest that this natural antimicrobial peptide could be a tool to develop new antibiotics.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Anti-Infective Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Antimicrobial Cationic Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/Glycine,
http://linkedlifedata.com/resource/pubmed/chemical/Indicators and Reagents,
http://linkedlifedata.com/resource/pubmed/chemical/Leucine
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| pubmed:status |
MEDLINE
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| pubmed:month |
Jul
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| pubmed:issn |
1879-3150
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| pubmed:author |
pubmed-author:BertoRaquel FRF,
pubmed-author:CamargoAntônio A C MAA,
pubmed-author:CardiBruno ABA,
pubmed-author:CarvalhoKrishnamurti MKM,
pubmed-author:Fontenele-CardiNauíla CNC,
pubmed-author:GoisElicélia AEA,
pubmed-author:HonórioJosé E RJEJr,
pubmed-author:KonnoKatsuhiroK,
pubmed-author:PimentaDaniel CDC,
pubmed-author:RichardsonMichaelM,
pubmed-author:RochaMarcos F GMF,
pubmed-author:SousaJuliana CJC
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| pubmed:issnType |
Electronic
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| pubmed:volume |
54
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
23-32
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| pubmed:meshHeading |
pubmed-meshheading:19298834-Amino Acid Sequence,
pubmed-meshheading:19298834-Animals,
pubmed-meshheading:19298834-Anti-Infective Agents,
pubmed-meshheading:19298834-Antimicrobial Cationic Peptides,
pubmed-meshheading:19298834-Bacteria,
pubmed-meshheading:19298834-Female,
pubmed-meshheading:19298834-Fungi,
pubmed-meshheading:19298834-Glycine,
pubmed-meshheading:19298834-Hemolysis,
pubmed-meshheading:19298834-Humans,
pubmed-meshheading:19298834-Indicators and Reagents,
pubmed-meshheading:19298834-Leucine,
pubmed-meshheading:19298834-Male,
pubmed-meshheading:19298834-Mass Spectrometry,
pubmed-meshheading:19298834-Microbial Sensitivity Tests,
pubmed-meshheading:19298834-Molecular Sequence Data,
pubmed-meshheading:19298834-Molecular Weight,
pubmed-meshheading:19298834-Ranidae,
pubmed-meshheading:19298834-Skin
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| pubmed:year |
2009
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| pubmed:articleTitle |
Leptoglycin: a new Glycine/Leucine-rich antimicrobial peptide isolated from the skin secretion of the South American frog Leptodactylus pentadactylus (Leptodactylidae).
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| pubmed:affiliation |
Laboratório de Toxinologia e Farmacologia Molecular, Instituto Superior de Ciências Biomédicas, Universidade Estadual do Ceará, Fortaleza, CE 60.740-000, Brazil.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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