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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
2009-3-18
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pubmed:abstractText |
We have developed a simple and effective method for surface modification of polymer microchips by entrapping hydroxypropyl cellulose (HPC) in a spin-coated thin film on the surface. Poly(methyl methacrylate-8.5-methacrylic acid), a widely available commercial resist formulation, was utilized as a matrix for dissolving HPC and providing adherence to native polymer surfaces. Various amounts of HPC (0.1-2.0%) dissolved in the copolymer and spun on polymer surfaces were evaluated. The modified surfaces were characterized by contact angle measurement, X-ray photoelectron spectroscopy and atomic force microscopy. The developed method was applied on both poly(methyl methacrylate) and cyclic olefin copolymer microchips. A fluorescently labeled myoglobin digest, binary protein mixture, and human serum sample were all separated in these surface-modified polymer microdevices. Our work exhibits an easy and reliable way to achieve favorable biomolecular separation performance in polymer microchips.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/19294306-10832882,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19294306-11504063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19294306-11811403,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19294306-12622396,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19294306-12628329,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19294306-12713054,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19294306-14613184,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19294306-14738849,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19294306-15283607,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19294306-15571346,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19294306-15924386,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19294306-15987099,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19294306-16372071,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19294306-16518374,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19294306-16738729,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19294306-16771530,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19294306-16960835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19294306-17594004,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19294306-18306184,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19294306-18633945
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Bicarbonates,
http://linkedlifedata.com/resource/pubmed/chemical/Buffers,
http://linkedlifedata.com/resource/pubmed/chemical/Cellulose,
http://linkedlifedata.com/resource/pubmed/chemical/Coated Materials, Biocompatible,
http://linkedlifedata.com/resource/pubmed/chemical/Composite Resins,
http://linkedlifedata.com/resource/pubmed/chemical/Cycloparaffins,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescein-5-isothiocyanate,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescent Dyes,
http://linkedlifedata.com/resource/pubmed/chemical/Methacrylates,
http://linkedlifedata.com/resource/pubmed/chemical/Myoglobin,
http://linkedlifedata.com/resource/pubmed/chemical/Polymers,
http://linkedlifedata.com/resource/pubmed/chemical/Polymethyl Methacrylate,
http://linkedlifedata.com/resource/pubmed/chemical/Spectrum composite resin,
http://linkedlifedata.com/resource/pubmed/chemical/hydroxypropylcellulose,
http://linkedlifedata.com/resource/pubmed/chemical/methacrylic acid
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
1473-0197
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
7
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pubmed:volume |
9
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
949-53
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pubmed:dateRevised |
2010-12-3
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pubmed:meshHeading |
pubmed-meshheading:19294306-Adsorption,
pubmed-meshheading:19294306-Animals,
pubmed-meshheading:19294306-Bicarbonates,
pubmed-meshheading:19294306-Buffers,
pubmed-meshheading:19294306-Cellulose,
pubmed-meshheading:19294306-Coated Materials, Biocompatible,
pubmed-meshheading:19294306-Composite Resins,
pubmed-meshheading:19294306-Cycloparaffins,
pubmed-meshheading:19294306-Fluorescein-5-isothiocyanate,
pubmed-meshheading:19294306-Fluorescent Dyes,
pubmed-meshheading:19294306-Horses,
pubmed-meshheading:19294306-Humans,
pubmed-meshheading:19294306-Hydrogen-Ion Concentration,
pubmed-meshheading:19294306-Methacrylates,
pubmed-meshheading:19294306-Microscopy, Atomic Force,
pubmed-meshheading:19294306-Myoglobin,
pubmed-meshheading:19294306-Polymers,
pubmed-meshheading:19294306-Polymethyl Methacrylate,
pubmed-meshheading:19294306-Serum,
pubmed-meshheading:19294306-Surface Properties,
pubmed-meshheading:19294306-Time Factors
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pubmed:year |
2009
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pubmed:articleTitle |
A general microchip surface modification approach using a spin-coated polymer resist film doped with hydroxypropyl cellulose.
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pubmed:affiliation |
Department of Chemistry and Biochemistry, Brigham Young University, Provo, Utah 84602-5700, USA.
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pubmed:publicationType |
Journal Article,
Evaluation Studies,
Research Support, N.I.H., Extramural
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