Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2009-8-5
pubmed:abstractText
We used minced, autologous skin for neoepithelialization of surgically created subcutaneous tunnels in a large animal model. Partial-thickness skin grafts were harvested from the back region of five 50-60 kg Yorkshire pigs. The skin was minced to 0.8 x 0.8 x 0.3 mm particles. Silicone-latex tubes were covered with fibrin, rolled in minced skin, and placed in subcutaneous tunnels created in the abdominal area. For comparison, single cell suspensions of keratinocytes and fibroblasts in fibrin or fibrin only were transplanted on tubes. Tunnels were extracted after 14, 21, and 28 days for microscopic evaluation. All tubes transplanted with minced skin particles showed neoepithelialization. The epithelium was stratified and differentiated after 2 weeks in vivo, and the stratum corneum was directed toward the implanted tube. No epithelium formed from tubes transplanted with single cell suspensions, and only sparse keratinocytes could be detected by serial sectioning and immunostaining on day 14, but not later. No epithelial lining was found in tunnels with fibrin-only-coated tubes. Epithelial cysts could be found the first 2 weeks after transplantation in the minced skin group but not later. In conclusion, a minced skin technique could serve as a potential source for tissue engineering of tubular conduits for reconstructive purposes of the urethra and for cutaneous stomas for bladder catheterization, or intestinal irrigations. The method would have the advantage of being simple and expeditious and not requiring in vitro culturing.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/19292681-11224923, http://linkedlifedata.com/resource/pubmed/commentcorrection/19292681-11678743, http://linkedlifedata.com/resource/pubmed/commentcorrection/19292681-11855913, http://linkedlifedata.com/resource/pubmed/commentcorrection/19292681-12447049, http://linkedlifedata.com/resource/pubmed/commentcorrection/19292681-1276671, http://linkedlifedata.com/resource/pubmed/commentcorrection/19292681-13571547, http://linkedlifedata.com/resource/pubmed/commentcorrection/19292681-1513896, http://linkedlifedata.com/resource/pubmed/commentcorrection/19292681-15371782, http://linkedlifedata.com/resource/pubmed/commentcorrection/19292681-15457038, http://linkedlifedata.com/resource/pubmed/commentcorrection/19292681-1584344, http://linkedlifedata.com/resource/pubmed/commentcorrection/19292681-16631879, http://linkedlifedata.com/resource/pubmed/commentcorrection/19292681-16846354, http://linkedlifedata.com/resource/pubmed/commentcorrection/19292681-17503306, http://linkedlifedata.com/resource/pubmed/commentcorrection/19292681-18947691, http://linkedlifedata.com/resource/pubmed/commentcorrection/19292681-4908835, http://linkedlifedata.com/resource/pubmed/commentcorrection/19292681-6379456, http://linkedlifedata.com/resource/pubmed/commentcorrection/19292681-7639486, http://linkedlifedata.com/resource/pubmed/commentcorrection/19292681-8833487, http://linkedlifedata.com/resource/pubmed/commentcorrection/19292681-9679932, http://linkedlifedata.com/resource/pubmed/commentcorrection/19292681-9817364, http://linkedlifedata.com/resource/pubmed/commentcorrection/19292681-9921652
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1937-335X
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
15
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2085-92
pubmed:dateRevised
2010-9-22
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Minced skin for tissue engineering of epithelialized subcutaneous tunnels.
pubmed:affiliation
Division of Pediatric Urology, Astrid Lindgren Children's Hospital, Karolinska University Hospital , Stockholm, Sweden.
pubmed:publicationType
Journal Article, Research Support, N.I.H., Extramural